Abstract | BACKGROUND: METHODS: RESULTS: The major findings of our study are: (1) CSF 5-MTHF, neopterin and biopterin concentrations are not affected by the ventriculo-spinal gradient; (2) pretreatment of CSF with ascorbic acid has negligible effects on 5-MTHF concentrations; (3) pretreatment of CSF with TCA/DTE and oxidation with iodine results in the most accurate determination of neopterin and biopterin; (4) when adjusted for age and total protein, CSF 5-MTHF correlated with 5-HIAA, but not with HVA; (5) the reference value of 5-MTHF in CSF in childhood is age-dependent (r=-0.634; p0.001); (6) we did not observe an age-dependency for neopterin and biopterin in CSF. CONCLUSION: 5-MTHF, neopterin and biopterin can be analysed in any volume of CSF that is collected. For correct analysis of pterins, CSF will have to be pretreated to stabilize the concentrations and stored properly, whereas such pretreatment is not necessary for 5-MTHF.
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Authors | M M Verbeek, A M Blom, R A Wevers, A J Lagerwerf, J van de Geer, M A A P Willemsen |
Journal | Molecular genetics and metabolism
(Mol Genet Metab)
Vol. 95
Issue 3
Pg. 127-32
(Nov 2008)
ISSN: 1096-7206 [Electronic] United States |
PMID | 18722797
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Tetrahydrofolates
- Biopterin
- Neopterin
- 5-methyltetrahydrofolate
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Topics |
- Adolescent
- Biopterin
(cerebrospinal fluid, chemistry)
- Child
- Child, Preschool
- Female
- Humans
- Infant
- Male
- Neopterin
(cerebrospinal fluid, chemistry)
- Nervous System Diseases
(cerebrospinal fluid, diagnosis)
- Specimen Handling
- Tetrahydrofolates
(cerebrospinal fluid, chemistry)
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