[Experimental studies of VEGF-C gene for the treatment of chronic obstructive lymphedema in mouse tail model].

Abstract

OBJECTIVE:

To study the therapeutic effect of vascular endothelial growth factor C (VEGF-C) gene for chronic obstructive lymphedema in mouse tail model which may provide a new treatment for lymphedema.

METHODS:

RT-PCR and immunoabsorption were applied to detect VEGF-C gene expression in fibroblasts and secretion of VEGF-C protein in COS7 cells respectively after pCDNA3.1 (+) VEGF-C transfection. A mouse tail model of chronic obstructive lymphedema was created. Then the pcDNA3.1-VEGF-C plasmid was injected into the tail. The effect of modulating lymphangiogenesis was observed.

RESULTS:

Compared with control group, overexpression of VEGF-C enhanced lymphangiogenesis in vivo and mouse tail skin suffering chronic obstructive lymphedema was improved by VEGF-C gene significantly.

CONCLUSIONS:

VEGF-C can improve the lymphedema through enhancing lymphangiogenesis.

• Authors: Xue-Qing Hu, Zhao-Hua Jiang, Ning-Fei Liu
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 24 Issue 3 Pg. 207-11 (May 2008) ISSN: 1009-4598 [Print] China
• PMID: 18717357 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Vascular Endothelial Growth Factor C

Topics

• Animals
• COS Cells
• Cells, Cultured
• Chlorocebus aethiops
• Disease Models, Animal
• Female
• Gene Transfer Techniques
• Genetic Therapy
• Humans
• Lymphedema (therapy)
• Mice
• Mice, Inbred BALB C
• Plasmids
• Transfection
• Vascular Endothelial Growth Factor C (genetics)