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Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice.

Abstract
Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.
AuthorsYasunori Sato, Shizuo Kajiyama, Akiko Amano, Yoshitaka Kondo, Toru Sasaki, Setsuko Handa, Ryoya Takahashi, Michiaki Fukui, Goji Hasegawa, Naoto Nakamura, Hikohito Fujinawa, Toyotaka Mori, Mitsuhiro Ohta, Hiroshi Obayashi, Naoki Maruyama, Akihito Ishigami
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 375 Issue 3 Pg. 346-50 (Oct 24 2008) ISSN: 1090-2104 [Electronic] United States
PMID18706888 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antioxidants
  • Calcium-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Rgn protein, mouse
  • Water
  • Superoxides
  • Hydrogen
  • Carboxylic Ester Hydrolases
  • gluconolactonase
  • Ascorbic Acid
Topics
  • Animals
  • Antioxidants (pharmacology)
  • Ascorbic Acid (metabolism)
  • Body Weight
  • Brain (drug effects, metabolism)
  • Calcium-Binding Proteins (genetics)
  • Carboxylic Ester Hydrolases (genetics)
  • Hydrogen (pharmacology)
  • Intracellular Signaling Peptides and Proteins (genetics)
  • Mice
  • Mice, Knockout
  • Models, Biological
  • Oxidative Stress
  • Superoxides (antagonists & inhibitors, metabolism)
  • Water (pharmacology)

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