Abstract | BACKGROUND:
Hepatocellular carcinoma (HCC) is characterized by multiple causes, clear multiple stages and a multifocal process of tumor progression related intimately to the overexpression of many cellular factors. The aim of this study was to investigate the dynamic expression of insulin-like growth factor-II ( IGF-II) and its abnormal alteration in the early stages of HCC development. METHODS:
Hepatoma models were induced by 2-fluorenylacetamide (2-FAA) in male Sprague-Dawley rats. Morphological changes of rat livers were assessed by pathological examination (HE staining). The levels of IGF-II expression in the livers and sera of rats were quantitatively detected by an enzyme-linked immunosorbent assay (ELISA). Simultaneously, the expression and cellular distribution of liver IGF-II were analyzed by immunohistochemistry. RESULTS: Histological examination confirmed that rat hepatocytes showed changes from granule-like degeneration to atypical hyperplasia to HCC, and progressively increasing hepatic IGF-II levels during HCC development. The levels of hepatic or serum IGF-II in HCC tissues and sera were significantly higher than those in normals and rats with degeneration. The immunohistochemical evidence indicated the positive expression and hepatocyte distribution of IGF-II in rat hepatoma. A positive relationship of IGF-II levels was found between liver tissues and sera of experimental rats (P<0.01). CONCLUSION: Hepatic IGF-II may participate in hepatocyte cancer development and detection of IGF-II expression during HCC development could be a useful molecular marker for its early diagnosis.
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Authors | Li-Wei Qiu, Deng-Fu Yao, Lei Zong, Yuan-Yuan Lu, Hua Huang, Wei Wu, Xin-Hua Wu |
Journal | Hepatobiliary & pancreatic diseases international : HBPD INT
(Hepatobiliary Pancreat Dis Int)
Vol. 7
Issue 4
Pg. 406-11
(Aug 2008)
ISSN: 1499-3872 [Print] Singapore |
PMID | 18693177
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Insulin-Like Growth Factor II
- 2-Acetylaminofluorene
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Topics |
- 2-Acetylaminofluorene
- Animals
- Biomarkers, Tumor
(blood, metabolism)
- Carcinoma, Hepatocellular
(chemically induced, metabolism, pathology)
- Cell Transformation, Neoplastic
(metabolism, pathology)
- Enzyme-Linked Immunosorbent Assay
- Immunohistochemistry
- Insulin-Like Growth Factor II
(metabolism)
- Liver Neoplasms, Experimental
(chemically induced, metabolism, pathology)
- Male
- Rats
- Time Factors
- Up-Regulation
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