Association of sterol regulatory element-binding protein-1c gene polymorphism with type 2 diabetes mellitus, insulin resistance and blood lipid levels in Chinese population.

Abstract

AIMS:

The sterol regulatory element-binding protein (SREBP)-1c gene has been identified as a susceptibility gene in metabolic diseases such as type 2 diabetes mellitus (T2DM), obesity, dyslipidemia and insulin resistance. Previous studies suggest that SNP17 (rs2297508, exon18c and G952G) of SREBP-1c gene and a common SREBP-1c SNP6 (rs11868035) are associated with an increased risk of T2DM. The present study aimed to confirm the previously reported association in a Chinese population and to examine the two SREBP-1c SNPs for their associations with insulin resistance and blood lipid.

METHODS:

We genotyped two SREBP-1c SNPs in a case-control study (n=327) from Chinese, including 156 patients with T2DM and 171 healthy controls, using polymerase chain reaction-denaturing high-performance liquid chromatography (PCR-DHPLC) and tested for association with type 2 diabetes, insulin resistance and blood lipid, respectively. Genotype and allele distributions and haplotype construction were analysed.

RESULTS:

The genotype and allele distributions of rs2297508 and rs11868035 polymorphisms were significantly different in type 2 diabetic patients compared to controls (P=0.002 and P=0.013; 0.00 and 0.001, respectively). Haplotype analyses showed significant association with diabetes risk and confirmed the results of the single SNP analyses. The plasma levels of LDL-c of the minor allele-C carriers of the two SNPs were both significantly higher than the noncarriers in the control group (P<0.05). Furthermore, insulin resistance index (HOMA-IRI) of the rare homozygotes C/C of rs11868035 was significantly lower than that of T/T in the T2DM group (P<0.05).

CONCLUSIONS:

These findings indicate that the SREBP-1c SNPs rs2297508 and rs11868035 are associated with a significantly increased risk of T2DM and dyslipidemia in the Chinese population. Moreover, the SNP (rs11868035) is closely related to insulin resistance (IR) in diabetic patients.

• Authors: Ju-Xiang Liu, Jing Liu, Pei-Qiang Li, Xiao-Dong Xie, Qian Guo, Li-Min Tian, Xiao-Qin Ma, Ji-Ping Zhang, Jia Liu, Jing-Yuan Gao
• Journal: Diabetes research and clinical practice (Diabetes Res Clin Pract) Vol. 82 Issue 1 Pg. 42-7 (Oct 2008) ISSN: 1872-8227 [Electronic] Ireland
• PMID: 18692268 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Sterol Regulatory Element Binding Protein 1

Topics

• Aged
• Asian People (genetics)
• China
• Diabetes Mellitus, Type 2 (ethnology, genetics)
• Female
• Gene Frequency
• Genotype
• Haplotypes
• Humans
• Insulin Resistance (genetics)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Sterol Regulatory Element Binding Protein 1 (genetics)