Abstract | BACKGROUND: PATIENTS AND METHODS: HAI-2 expression was examined in cervical cancer specimens (n=52) by immunohistochemistry. We further attempted to investigate the biological functions and inhibitory effects of HAI-2 using human papillomavirus (HPV) 16 type SiHa and HPV 18 type HeLa cervical cancer cell lines. RESULTS: There were significant correlations between HAI-2 expression and stage (P=0.017), lymph node metastasis (P=0.005) and ovarian metastasis (P=0.038). Low HAI-2 expression was a significant predictor for a poor prognosis compared with high HAI-2 expression (disease-free survival rate, P=0.016; overall survival rate, P=0.021). After transient transfection into the SiHa and HeLa cell lines, HAI-2 showed potential inhibitory effects mediated by reductions in hepsin and matriptase expression, which led to apoptosis by increasing the level of Bak and reducing the level of Bcl-2. CONCLUSIONS: The present findings indicate that low HAI-2 expression in cervical cancer may be associated with a poor prognosis. We propose that HAI-2 may represent a therapeutic target for the treatment of cervical cancer.
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Authors | K Nakamura, F Abarzua, A Hongo, J Kodama, Y Nasu, H Kumon, Y Hiramatsu |
Journal | Annals of oncology : official journal of the European Society for Medical Oncology
(Ann Oncol)
Vol. 20
Issue 1
Pg. 63-70
(Jan 2009)
ISSN: 1569-8041 [Electronic] England |
PMID | 18689863
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Membrane Glycoproteins
- SPINT2 protein, human
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Topics |
- Adenocarcinoma
(diagnosis, genetics, metabolism, pathology)
- Adult
- Aged
- Apoptosis
(genetics)
- Biomarkers, Tumor
(genetics, metabolism, physiology)
- Carcinoma, Adenosquamous
(diagnosis, genetics, metabolism, pathology)
- Carcinoma, Squamous Cell
(diagnosis, genetics, metabolism, pathology)
- Cell Proliferation
- Female
- HeLa Cells
- Humans
- Membrane Glycoproteins
(genetics, metabolism, physiology)
- Middle Aged
- Prognosis
- Signal Transduction
(genetics)
- Transfection
- Tumor Cells, Cultured
- Uterine Cervical Neoplasms
(diagnosis, genetics, metabolism, pathology)
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