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Fusion of enhanced green fluorescent protein to the pseudorabies virus axonal sorting protein Us9 blocks anterograde spread of infection in mammalian neurons.

Abstract
Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.
AuthorsM G Lyman, D Curanovic, A D Brideau, L W Enquist
JournalJournal of virology (J Virol) Vol. 82 Issue 20 Pg. 10308-11 (Oct 2008) ISSN: 1098-5514 [Electronic] United States
PMID18684822 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Intracellular Signaling Peptides and Proteins
  • Lipoproteins
  • Phosphoproteins
  • Recombinant Fusion Proteins
  • US9 protein, Suid herpesvirus 1
  • Viral Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
Topics
  • Animals
  • Axons (metabolism)
  • Brain (cytology, metabolism)
  • Cells, Cultured
  • Green Fluorescent Proteins (genetics, metabolism)
  • Herpesvirus 1, Suid (genetics, metabolism)
  • Intracellular Signaling Peptides and Proteins
  • Lipoproteins (genetics, metabolism)
  • Male
  • Membrane Microdomains (metabolism)
  • Neurons (cytology, metabolism, virology)
  • Phosphoproteins (genetics, metabolism)
  • Protein Transport
  • Pseudorabies (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins (genetics, metabolism)
  • Viral Proteins (genetics, metabolism)

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