Abstract |
Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.
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Authors | M G Lyman, D Curanovic, A D Brideau, L W Enquist |
Journal | Journal of virology
(J Virol)
Vol. 82
Issue 20
Pg. 10308-11
(Oct 2008)
ISSN: 1098-5514 [Electronic] United States |
PMID | 18684822
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Intracellular Signaling Peptides and Proteins
- Lipoproteins
- Phosphoproteins
- Recombinant Fusion Proteins
- US9 protein, Suid herpesvirus 1
- Viral Proteins
- enhanced green fluorescent protein
- Green Fluorescent Proteins
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Topics |
- Animals
- Axons
(metabolism)
- Brain
(cytology, metabolism)
- Cells, Cultured
- Green Fluorescent Proteins
(genetics, metabolism)
- Herpesvirus 1, Suid
(genetics, metabolism)
- Intracellular Signaling Peptides and Proteins
- Lipoproteins
(genetics, metabolism)
- Male
- Membrane Microdomains
(metabolism)
- Neurons
(cytology, metabolism, virology)
- Phosphoproteins
(genetics, metabolism)
- Protein Transport
- Pseudorabies
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Recombinant Fusion Proteins
(genetics, metabolism)
- Viral Proteins
(genetics, metabolism)
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