cDNA clone, prokaryotic expression and purification of human interleukin-13 receptor [alpha]2 chain.

Abstract

Despite advances in surgical technology and radiation therapy, the prognosis in the patients with malignant glioma remains poor. Recent studies show that interleukin-13 receptor [alpha]2 chain (IL-13Ra2), a brain tumor-associated receptor for IL-13, may play a role in immunotherapy for glioblastoma. We thus amplified human IL-13Ra2 gene from the human glioblastoma cell line using RT-PCR and cloned the target gene into the pET-28a, a prokaryotic expressing plasmid. After transformation, the recombinant plasmid expressed a soluble protein induced by IPTG. The purified recombinant protein was shown to be a single band on the SDS-PAGE with a predicated molecular weight of human IL-13Ra2 gene, suggesting that the recombinant protein of human IL-13Ra2 was successfully expressed. Recombinant IL-13Ra2 protein can be used as an anti-tumor vaccine, which may provide a promising new strategy for the treatment of brain malignant gliomas.

• Authors: Wenqiao Yu, Zhipeng Su, Zhebao Wu, Xiaochun Mao, Weiming Zheng, Yanjun Zeng
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 58 Issue 3 Pg. 409-13 (Mar 2009) ISSN: 1432-0851 [Electronic] Germany
• PMID: 18677476 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• DNA, Complementary
• Interleukin-13 Receptor alpha2 Subunit

Topics

• Antigens, Neoplasm (chemistry)
• Brain Neoplasms (metabolism)
• Cell Line, Tumor
• Cloning, Molecular
• DNA, Complementary (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Gene Expression Regulation, Neoplastic
• Glioma (metabolism)
• Humans
• Immunotherapy (methods)
• Interleukin-13 Receptor alpha2 Subunit (genetics, metabolism)
• Models, Biological
• Molecular Weight
• Plasmids (metabolism)
• Time Factors