Abstract |
Hydrogen sulfide (H(2)S) is an endogenous gaseous mediator with the ability to modulate tissue inflammation and pain. The aim of this study was to determine the effect of an H(2)S donor (Na(2)S) on leukocyte-endothelium interactions, blood flow, and pain sensation in acutely inflamed knee joints. Acute arthritis was induced in urethane anesthetized C57bl/6 mice by intra-articular injection of kaolin/ carrageenan (24-h recovery), and the effect of local administration of Na(2)S on leukocyte trafficking was measured by intravital microscopy. Synovial blood flow was measured in inflamed knees by laser Doppler perfusion imaging. Finally, the effect of an intra-articular injection of Na(2)S on joint pain in control and inflamed rats was determined by hindlimb incapacitance and von Frey hair algesiometry. Local administration of an H(2)S donor to inflamed knees caused a dose-dependent reduction in leukocyte adherence and an increase in leukocyte velocity. These effects could be inhibited by coadministration of the ATP-sensitive K(+) channel blocker glibenclamide. Local administration of Na(2)S to inflamed joints caused a pronounced vasoconstrictor response; however, there was no observable effect of Na(2)S on joint pain. These findings establish H(2)S as a novel signaling molecule in rodent knee joints. H(2)S exhibits potent anti-inflammatory properties, but with no detectable effect on joint pain.
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Authors | Benjamin Andruski, Donna-Marie McCafferty, Teegan Ignacy, Brandie Millen, Jason J McDougall |
Journal | American journal of physiology. Regulatory, integrative and comparative physiology
(Am J Physiol Regul Integr Comp Physiol)
Vol. 295
Issue 3
Pg. R814-20
(Sep 2008)
ISSN: 0363-6119 [Print] United States |
PMID | 18667709
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Sulfides
- Kaolin
- Carrageenan
- sodium bisulfide
- Hydrogen Sulfide
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Topics |
- Acute Disease
- Animals
- Arthritis
(chemically induced, drug therapy, immunology)
- Behavior, Animal
(physiology)
- Carrageenan
(pharmacology)
- Cell Movement
(drug effects, immunology)
- Hydrogen Sulfide
(metabolism)
- Kaolin
(pharmacology)
- Knee Joint
(blood supply, immunology)
- Leukocytes
(cytology, immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Pain
(chemically induced, drug therapy, immunology)
- Physical Stimulation
- Proprioception
(physiology)
- Signal Transduction
(drug effects, immunology)
- Sulfides
(pharmacology)
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