A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-57) with cytotoxic and apoptotic effects against human cancer cells.

Abstract

A series of novel peptides from various motifs of Asterina pectinifera cyclin B and their derivatives conjugated to HIV-Tat(49-57) were designed and synthesized. Their bioactivities on two human cancer cell lines were determined. Among them, Tat-a5 (KAQIRAMECNILGRKKRRQRRR) exhibited significant cytotoxic effects on cancer cell lines EC-9706 and HCT-116. Tat-a5 could arrest cancer cells at G(2)/M phase and make them apoptotic. Our results suggested that Tat-a5 could be a novel leading peptide with anticancer activity.

• Authors: Huiping Lou, Yanfeng Gao, Mingxia Zhai, Yuanming Qi, Lixiang Chen, Hong Lv, Jibing Yu, Yongxin Li
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 18 Issue 16 Pg. 4633-7 (Aug 15 2008) ISSN: 1464-3405 [Electronic] England
• PMID: 18656352 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Cyclin B
• Peptide Fragments
• Peptides
• tat Gene Products, Human Immunodeficiency Virus
• tat peptide (49-57), Human immunodeficiency virus 1

Topics

• Amino Acid Sequence
• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis
• Asterina (metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Chemistry, Pharmaceutical (methods)
• Cyclin B (chemistry)
• Dose-Response Relationship, Drug
• Drug Design
• Humans
• Molecular Sequence Data
• Peptide Fragments (chemistry)
• Peptides (chemistry)
• tat Gene Products, Human Immunodeficiency Virus (chemistry)