A natural human IgM antibody that neutralizes botulinum neurotoxin in vivo.

Abstract

Affinity-matured human antibodies have demonstrated efficacy as countermeasures for exposure to botulinum neurotoxin (BoNT), which is the cause of the disease botulism category A select bioterror agent. Little is known, however, about the potential role of natural (un-mutated) antibodies in the protective immune response to BoNT. Here we describe the cloning of two human IgM antibodies that bind serotype A BoNT. Both are un-mutated IgM antibodies, consistent with an origin in naive B cells. One of the antibodies is able to fully neutralize a lethal dose of serotype A BoNT in vivo. These results suggest that the natural human antibody repertoire may play a role in protection from exposure to biological toxins.

• Authors: Sharad P Adekar, Fetweh H Al-Saleem, M D Elias, Katherine A Rybinski, Lance L Simpson, Scott K Dessain
• Journal: Hybridoma (2005) (Hybridoma (Larchmt)) Vol. 27 Issue 2 Pg. 65-9 (Apr 2008) ISSN: 1554-0014 [Print] United States
• PMID: 18642670 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antibodies, Bacterial
• Immunoglobulin M
• rimabotulinumtoxinB
• Botulinum Toxins
• Botulinum Toxins, Type A

Topics

• Amino Acid Sequence
• Antibodies, Bacterial (administration & dosage, metabolism, physiology)
• Binding Sites, Antibody
• Botulinum Toxins (antagonists & inhibitors, immunology, metabolism)
• Botulinum Toxins, Type A (antagonists & inhibitors, immunology, metabolism)
• Botulism (immunology, prevention & control)
• Cell Line, Tumor
• Cells, Cultured
• Clostridium botulinum (immunology)
• Humans
• Hybridomas
• Immunoglobulin M (administration & dosage, metabolism, physiology)
• Molecular Sequence Data