Abstract |
We propose that elevation of mitochondrial enzyme cofactors may prevent or ameliorate neurodegenerative diseases by improving mitochondrial function. In the present study, we investigated the effects of high doses of B vitamins, the precursors of mitochondrial enzyme cofactors, on mitochondrial dysfunction, oxidative stress, and Parkinsonism in a 4-week long rotenone treatment-induced cellular model of Parkinson's disease (PD). Pretreatment with B vitamins (also 4 weeks) prevented rotenone-induced: (1) mitochondrial dysfunction, including reduced mitochondrial membrane potential and activities of complex I; (2) oxidative stress, including increase in reactive oxygen species, oxidative DNA damage and protein oxidation, and (3) Parkinsonism parameters, including accumulation of alpha-synuclein and poly- ubiquitin. The optimum doses were found around 2.5- and 5-fold of that in normal MEM medium. The 4-week pretreatment was chosen based on time-dependent experiments that pretreatments longer than 2 weeks resulted in a decrease in oxidants, an increase in oxygen consumption, and up-regulation of complex I activity and PGC-1alpha expression. Individual B vitamins at the same doses did not show a similar effect suggesting that these B vitamins work synergistically. These results suggest that administration of high doses of B vitamins sufficient to elevate mitochondrial enzyme cofactors may be effective in preventing PD by reducing oxidative stress and improving mitochondrial function.
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Authors | Haiqun Jia, Zhongbo Liu, Xin Li, Zhihui Feng, Jiejie Hao, Xuesen Li, Weili Shen, Hongyu Zhang, Jiankang Liu |
Journal | Neurobiology of aging
(Neurobiol Aging)
Vol. 31
Issue 4
Pg. 636-46
(Apr 2010)
ISSN: 1558-1497 [Electronic] United States |
PMID | 18639366
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2008 Elsevier Inc. All rights reserved. |
Chemical References |
- Biomarkers
- Coenzymes
- Heat-Shock Proteins
- Nerve Tissue Proteins
- PPARGC1A protein, human
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
- Reactive Oxygen Species
- Transcription Factors
- Ubiquitin
- Uncoupling Agents
- alpha-Synuclein
- Rotenone
- Vitamin B Complex
- Electron Transport Complex I
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Topics |
- Biomarkers
- Cell Line, Tumor
- Coenzymes
(metabolism, pharmacology, therapeutic use)
- DNA Damage
(drug effects, physiology)
- Dose-Response Relationship, Drug
- Drug Administration Schedule
- Drug Synergism
- Electron Transport Complex I
(drug effects, metabolism)
- Heat-Shock Proteins
(drug effects, metabolism)
- Humans
- Membrane Potential, Mitochondrial
(drug effects, physiology)
- Mitochondria
(drug effects, metabolism)
- Mitochondrial Diseases
(drug therapy, metabolism, physiopathology)
- Models, Biological
- Nerve Tissue Proteins
(drug effects, metabolism)
- Oxidative Stress
(drug effects, physiology)
- Oxygen Consumption
(drug effects, physiology)
- Parkinson Disease
(drug therapy, metabolism, physiopathology)
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
- Reactive Oxygen Species
(antagonists & inhibitors, metabolism)
- Rotenone
(antagonists & inhibitors, toxicity)
- Transcription Factors
(drug effects, metabolism)
- Ubiquitin
(antagonists & inhibitors, metabolism)
- Uncoupling Agents
(antagonists & inhibitors, toxicity)
- Vitamin B Complex
(metabolism, pharmacology, therapeutic use)
- alpha-Synuclein
(antagonists & inhibitors, metabolism)
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