Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays.

Abstract

We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant Mycobacterium tuberculosis. A total of 151 M. tuberculosis strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Löwenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.

• Authors: Dissou Affolabi, N'Dira Sanoussi, Mathieu Odoun, Anandi Martin, Louis Koukpemedji, Juan Carlos Palomino, Luc Kestens, Séverin Anagonou, Françoise Portaels
• Journal: Journal of medical microbiology (J Med Microbiol) Vol. 57 Issue Pt 8 Pg. 1024-1027 (Aug 2008) ISSN: 0022-2615 [Print] England
• PMID: 18628506 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Nitrate Reductase
• Rifampin

Topics

• Bacterial Proteins (metabolism)
• Benin
• Colorimetry
• Drug Resistance, Bacterial
• Drug Resistance, Multiple (physiology)
• Humans
• Mycobacterium tuberculosis (drug effects, enzymology, isolation & purification)
• Nitrate Reductase (metabolism)
• Rifampin (pharmacology)