Contamination levels of perfluorinated compounds (PFCs), including
perfluorooctanesulfonate (PFOS),
perfluorononanoic acid (PFNA),
perfluorooctane sulfonamide (
PFOSA), perfluorohexanesulfonate (PFHS), and
perfluorooctanoic acid (PFOA), were determined in the livers of wild common cormorants (Phalacrocorax carbo) from Lake Biwa, Japan. Potential effects of PFCs alone and combined effects by
complex mixture of PFCs and
dioxins and related compounds (DRCs) were also assessed by gene expression profiling using a cormorant oligo array.
Perfluorooctanesulfonate, PFNA, and
PFOSA were detected in almost all liver samples analyzed, while concentrations of PFHS and PFOA were below the limit of quantification. The microarray data analyses revealed that hepatic PFC levels were correlated with the expression of 74 genes. Real-time reverse-transcript polymerase chain reaction data demonstrated that PFOS concentration was positively correlated with
mRNA levels of
glutathione peroxidase 1 and
glutathione S-transferase alpha 3 and negatively correlated with levels of heat shock 70-kDa
protein 8 and
tumor rejection
antigen 1 mRNAs. These results suggest the induction of
antioxidant enzymes in response to oxidative stress caused by PFCs and the suppression of
molecular chaperones, leading to reduction in protein stability. Moreover, multiple regression analyses identified seven significant models in which certain genes showed expression levels altered by accumulation of PFCs and DRCs. The regression models explained associations with
cytochrome P450 1A
mRNA and
protein expression levels, and its catalytic activity,
ethoxyresorufin-O-deethylase of both PFNA and the
2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent levels. Thus, the regression models suggested the potential of PFCs to enhance toxicities of DRCs. Since mixture toxicity is an urgent issue, further study is required to understand the effects of mixtures of PFCs and DRCs in wild cormorants.