Abstract |
In the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice. We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides ( CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-gamma response mediated mainly by CD4(+) T cell, albeit a minor contribution of CD8(+) T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-gamma production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan- Leishmania vaccine.
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Authors | Salvador Iborra, Nuria Parody, Daniel R Abánades, Pedro Bonay, Deboraci Prates, Fernanda O Novais, Manoel Barral-Netto, Carlos Alonso, Manuel Soto |
Journal | Microbes and infection
(Microbes Infect)
2008 Aug-Sep
Vol. 10
Issue 10-11
Pg. 1133-41
ISSN: 1286-4579 [Print] France |
PMID | 18603012
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CPG-oligonucleotide
- Immunoglobulin G
- Leishmaniasis Vaccines
- Oligodeoxyribonucleotides
- Protozoan Proteins
- Ribosomal Proteins
- Interleukin-10
- Interleukin-4
- Interferon-gamma
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Topics |
- Animals
- Female
- Immunoglobulin G
(metabolism)
- Interferon-gamma
(metabolism)
- Interleukin-10
(metabolism)
- Interleukin-4
(metabolism)
- Leishmania major
(immunology)
- Leishmaniasis Vaccines
- Leishmaniasis, Cutaneous
(immunology, prevention & control)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Oligodeoxyribonucleotides
(genetics, immunology, metabolism)
- Protozoan Proteins
(genetics, immunology, metabolism)
- Ribosomal Proteins
(genetics, immunology, metabolism)
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