Influence of local tetracycline on the microbiota of alveolar osteitis in rats.

Abstract

The aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. Forty Wistar rats were randomly assigned to 4 groups (n=10): I - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; II - adrenaline and Ringer-PRAS were introduced into the alveolar wound; III - the alveolar wound was irrigated with sterile saline; and IV - the alveolar wound was irrigated with an aqueous solution of tetracycline. Microbial samples from the alveolar wounds were collected 2 days after surgery and inoculated on blood agar (with and without 8 microg/mL of tetracycline) and other selective media, and were incubated in either aerobiosis or anaerobiosis at 37 degrees C, for 2 to 14 days. It was verified that tetracycline reduced the occurrence of alveolar osteitis in the rats and caused significant changes in the microbiota of the surgical sites, decreasing the number of anaerobes and increasing the participation of tetracycline-resistant and multi-resistant microorganisms.

• Authors: Joseane Maria Dias Bosco, Sérgio Ricardo de Oliveira, Alvaro Francisco Bosco, Christiane Marie Schweitzer, Elerson Gaetti Jardim Júnior
• Journal: Brazilian dental journal (Braz Dent J) Vol. 19 Issue 2 Pg. 119-23 ( 2008) ISSN: 1806-4760 [Electronic] Brazil
• PMID: 18568225 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Isotonic Solutions
• Vasoconstrictor Agents
• Ringer's Solution
• Tetracycline
• Epinephrine

Topics

• Actinomyces (drug effects)
• Animals
• Anti-Bacterial Agents (therapeutic use)
• Bacteroides (drug effects)
• Colony Count, Microbial
• Drug Resistance, Multiple, Bacterial
• Dry Socket (microbiology, prevention & control)
• Enterobacteriaceae (drug effects)
• Enterococcus (drug effects)
• Epinephrine (therapeutic use)
• Eubacterium (drug effects)
• Fusobacterium (drug effects)
• Incisor (surgery)
• Isotonic Solutions
• Male
• Peptostreptococcus (drug effects)
• Random Allocation
• Rats
• Rats, Wistar
• Ringer's Solution
• Suppuration
• Tetracycline (therapeutic use)
• Tetracycline Resistance
• Therapeutic Irrigation
• Tooth Extraction
• Tooth Socket (drug effects, microbiology)
• Vasoconstrictor Agents (therapeutic use)
• Veillonella (drug effects)