Abstract | BACKGROUND/PURPOSE: Congenital cystic adenomatoid malformation (CCAM) of the lung may result from a localized aberrant epithelial-mesenchymal interaction during lung development. We used laser microdissection (LMD) to isolate the epithelium and mesenchyme of CCAM, and studied candidate gene expression in these pure cell populations. METHODS: Congenital cystic adenomatoid malformation tissue was obtained from fetal (n = 5) and postnatal (n = 5) surgical specimens. Normal fetal lung (n = 10) was obtained from abortus material, and normal postnatal lung (n = 5) was identified from surgical specimens. Whole tissue was analyzed using immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). Using LMD, columnar bronchiolar type epithelium and underlying mesenchyme were isolated. Multiplex nested RT-PCR was then used to detect message levels of candidate genes. RESULTS: Reverse transcriptase polymerase chain reaction performed on LMD-isolated tissue, but not whole tissue homogenate, revealed differences between CCAM and normal lung. In this report, we focus on the fibroblast growth factor (FGF) family. By RT-PCR, there was 4-fold more epithelial expression of FGF9 in fetal CCAM vs normal fetal lung (P < .07). This was qualitatively confirmed by immunohistochemistry. We also detected decreased FGF7 expression in CCAM mesenchyme (P < .05) but no significant differences in FGF10 or FGFR2. CONCLUSIONS: LMD may be used to overcome the limitations of tissue heterogeneity in the study of CCAM. Abnormal growth factor expression may play a role in the etiology of this lesion.
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Authors | Tim Jancelewicz, Kerilyn Nobuhara, Sam Hawgood |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 43
Issue 6
Pg. 1044-51
(Jun 2008)
ISSN: 1531-5037 [Electronic] United States |
PMID | 18558180
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- FGF10 protein, human
- Fibroblast Growth Factor 10
- Fibroblast Growth Factor 9
- Fibroblast Growth Factor 7
- Fibroblast Growth Factors
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Topics |
- Child
- Cohort Studies
- Cystic Adenomatoid Malformation of Lung, Congenital
(genetics, pathology)
- Female
- Fetus
(pathology)
- Fibroblast Growth Factor 10
(genetics)
- Fibroblast Growth Factor 7
(genetics)
- Fibroblast Growth Factor 9
(genetics)
- Fibroblast Growth Factors
(genetics)
- Gene Expression Regulation, Developmental
- Genetic Predisposition to Disease
- Humans
- Immunohistochemistry
- In Situ Nick-End Labeling
- Infant
- Infant, Newborn
- Lasers
- Male
- Microdissection
(instrumentation, methods)
- Reference Values
- Reverse Transcriptase Polymerase Chain Reaction
- Risk Assessment
- Sensitivity and Specificity
- Tissue Culture Techniques
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