Potential advantages of cell administration on the inflammatory response compared to standard ACE inhibitor treatment in experimental myocardial infarction.

Abstract

BACKGROUND:

Bone Marrow (BM) progenitor cells can target the site of myocardial injury, contributing to tissue repair by neovascolarization and/or by a possible direct paracrine effect on the inflammatory cascade. Angiotensin Converting Enzyme inhibitors (ACE-I) are effective in reducing mortality and preventing left ventricular (LV) function deterioration after myocardial infarction.

METHODS:

We investigated the short term effects of BM mononuclear cells (BMMNCs) therapy on the pro-inflammatory cytokines (pro-CKs) and on LV remodelling and compared these effects over a standard ACE-I therapy in a rat model of myocardial cryodamage. Forty two adult inbread Fisher-F344 rats were randomized into three groups: untreated (UT; n = 12), pharmacological therapy (ACE-I; n = 14, receiving quinapril), and cellular therapy (BMMNCs; n = 16, receiving BMMNCs infusion). Rats underwent to a standard echocardiogram in the acute setting and 14 days after the damage, before the sacrifice. Pro-CKs analysis (interleukin (IL)1beta, IL-6, tumor necrosis factor (TNF)alpha was performed (multiplex proteome arrays) on blood samples obtained by direct aorta puncture before the sacrifice; a control group of 6 rats was considered as reference.

RESULTS:

Concerning the extension of the infarcted area as well as the LV dimensions, no differences were observed among the animal groups; treated rats had lower left atrial diameters and higher indexes of LV function. Pro-Cks were increased in infarcted-UT rats if compared with controls, and significantly reduced by BMMNCs and ACE-I ; TNFalpha inversely correlated with LV fractional shortening.

CONCLUSION:

After myocardial infarction, both BMMNCs and ACE-I reduce the pattern of pro-Ck response, probably contributing to prevent the deterioration of LV function observed in UT rats.

• Authors: Michele M Ciulla, Elisa Montelatici, Stefano Ferrero, Paola Braidotti, Roberta Paliotti, Giuseppe Annoni, Elisa De Camilli, Giuseppe Busca, Luisa Chiappa, Paolo Rebulla, Fabio Magrini, Lorenza Lazzari
• Journal: Journal of translational medicine (J Transl Med) Vol. 6 Pg. 30 (Jun 12 2008) ISSN: 1479-5876 [Electronic] England
• PMID: 18549470 (Publication Type: Comparative Study, Evaluation Study, Journal Article)

Chemical References

• Angiotensin-Converting Enzyme Inhibitors
• Tumor Necrosis Factor-alpha

Topics

• Algorithms
• Angiotensin-Converting Enzyme Inhibitors (therapeutic use)
• Animals
• Bone Marrow Transplantation (immunology, physiology)
• Disease Models, Animal
• Heart Ventricles (pathology)
• Inflammation (etiology, therapy)
• Male
• Myocardial Infarction (blood, complications, pathology, therapy)
• Random Allocation
• Rats
• Rats, Inbred F344
• Tumor Necrosis Factor-alpha (blood)