Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus.

Abstract

Exosomes have been proposed as candidates for therapeutic immunization. The present study demonstrates that incorporation of the G protein of vesicular stomatitis virus (VSV-G) into exosome-like vesicles (ELVs) enhances their uptake and induces the maturation of dendritic cells. Targeting of VSV-G and ovalbumin as a model antigen to the same ELVs increased the cross-presentation of ovalbumin via an endosomal acidification mechanism. Immunization of mice with VSV-G and ovalbumin containing ELVs led to an increased IgG2a antibody response, expansion of antigen-specific CD8 T cells, strong in vivo CTL responses, and protection from challenge with ovalbumin expressing tumor cells. Thus, incorporation of VSV-G and targeting of antigens to ELVs are attractive strategies to improve exosomal vaccines.

• Authors: Vladimir V Temchura, Matthias Tenbusch, Godwin Nchinda, Ghulam Nabi, Bettina Tippler, Maryna Zelenyuk, Oliver Wildner, Klaus Uberla, Seraphin Kuate
• Journal: Vaccine (Vaccine) Vol. 26 Issue 29-30 Pg. 3662-72 (Jul 04 2008) ISSN: 0264-410X [Print] Netherlands
• PMID: 18538453 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• G protein, vesicular stomatitis virus
• Membrane Glycoproteins
• Vaccines
• Viral Envelope Proteins
• Ovalbumin

Topics

• Animals
• Antibodies (blood)
• Antigen Presentation
• CD8-Positive T-Lymphocytes (immunology)
• Cytotoxicity, Immunologic
• Dendritic Cells (immunology)
• Endosomes (metabolism)
• Humans
• Membrane Glycoproteins (immunology)
• Mice
• Mice, Inbred C57BL
• Neoplasms (prevention & control)
• Ovalbumin (immunology)
• Protein Transport
• Secretory Vesicles (immunology)
• Vaccines (immunology)
• Viral Envelope Proteins (immunology)