Abstract | BACKGROUND: Determination of drug penetration in diseased skin represents a challenge. OBJECTIVE: To compare dermal microdialysis and tape-strip sampling of drug penetration in normal skin and skin with irritant dermatitis. METHODS: The two methodologies were employed simultaneously in 16 healthy volunteers. Samples were collected in a study of the penetration of a metronidazole cream formulation ( Flagyl 1%) applied to forearm skin in both areas with irritant dermatitis and normal skin. Barrier perturbation and the depth of microdialysis probes were quantified by non-invasive bioengineering methods. RESULTS: CONCLUSION: The selection of sampling methodology should be based on the skin layer of interest as well as the integrity of the skin barrier. Whenever the dermal tissue is the target for topical treatment, microdialysis sampling should be the method of choice.
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Authors | Patricia García Ortiz, Steen Honoré Hansen, Vinod P Shah, Torkil Menné, Eva Benfeldt |
Journal | Contact dermatitis
(Contact Dermatitis)
Vol. 59
Issue 1
Pg. 23-30
(Jul 2008)
ISSN: 1600-0536 [Electronic] England |
PMID | 18537995
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dermatologic Agents
- Metronidazole
- Sodium Dodecyl Sulfate
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Topics |
- Administration, Cutaneous
- Adult
- Analysis of Variance
- Area Under Curve
- Biological Availability
- Dermatitis, Irritant
(metabolism)
- Dermatologic Agents
(administration & dosage, pharmacokinetics)
- Erythema
(chemically induced)
- Female
- Humans
- Linear Models
- Male
- Metronidazole
(administration & dosage, pharmacokinetics)
- Microdialysis
(methods)
- Middle Aged
- Skin
(diagnostic imaging, injuries)
- Skin Absorption
- Sodium Dodecyl Sulfate
- Ultrasonography
- Water Loss, Insensible
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