Abstract |
Previously, our group has used a B16-F10 melanoma model to show that C-C chemokine receptor 5 (CCR5) knockout (CCR5(-/-)) mice form fewer pulmonary metastases than wild-type mice. This advantage can be eliminated by injecting CCR5(-/-) mice with wild-type pulmonary mesenchymal cells before tumor injection. In this article, we present the mechanisms underlying this finding. First, we demonstrate that wild-type mesenchymal cells migrate to CCL4 more efficiently in vitro than CCR5(-/-) cells. Wild-type mesenchymal cells were also 3.6 (1.85 to 5.85) times more efficient than CCR5(-/-) cells at migrating into the lung after intravenous injection (P < 0.01). The injection of wild-type but not CCR5(-/-) mesenchymal cells led to a 7.0 +/- 1.6 (P < 0.05)-fold induction of matrix metalloproteinase 9 (MMP9) in the host lung. Neither wild-type nor CCR5(-/-) cells caused significant increases in MMP2, MMP3, or MMP8. Inhibition of the gelatinase activity of MMP9 decreased the number of metastases and restored the advantage that CCR5(-/-) mice have over wild-type mice. Further analysis showed that the CCR5(+) mesenchymal cells expressed CD45(+) and CD13(+) but did not express alpha-smooth muscle actin. This phenotype is characteristic of a subset of mesenchymal cells called fibrocytes. Together, these data suggest a novel role for CCR5 in the migration of pulmonary fibrocytes and the promotion of metastasis.
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Authors | Hendrik W van Deventer, Qing Ping Wu, Daniel T Bergstralh, Beckley K Davis, Brian P O'Connor, Jenny P-Y Ting, Jonathan S Serody |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 173
Issue 1
Pg. 253-64
(Jul 2008)
ISSN: 1525-2191 [Electronic] United States |
PMID | 18535183
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Receptors, CCR5
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Blotting, Western
- Cell Movement
(physiology)
- Connective Tissue Cells
(metabolism)
- Flow Cytometry
- Immunohistochemistry
- Lung Neoplasms
(secondary)
- Matrix Metalloproteinase 9
(metabolism)
- Melanoma, Experimental
(pathology)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Neoplasm Invasiveness
(pathology)
- Neoplasm Metastasis
(pathology)
- Oligonucleotide Array Sequence Analysis
- Receptors, CCR5
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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