Abstract | BACKGROUND: METHODS: Diabetes was induced in male Wistar rats by intraperitonal injection of streptozotocin (55 mg/kg). Some of the non-diabetic (control) and diabetic rats treated with STB (24.7 mg/kg/day) during 16 weeks, and the effects on blood glucose, PC, AOPP, 3-NT, P-SH, T-SH and Np-SH were studied. Biomarkers were assayed by enzyme-linked immunosorbent assay (ELISA) or by colorimetric methods. RESULTS: Administration of stobadine to diabetic animals lowered elevated blood glucose levels by approximately 16% relative to untreated diabetic rats. Although stobadine decreased blood glucose, poor glycemic control was maintained in stobadine treated diabetic rats during the treatment period. Biochemical analyses of liver proteins showed significant diminution of sulfhydryl groups, P-SH, T-SH, Np-SH, and elevation of carbonyl groups in diabetic animals in comparison to healthy controls. As a biomarker of nitrosative stress, 3-NT levels did not significantly change by diabetes induction or by stobadine treatment when compared to control animals. However, the treatment with stobadine resulted in a significant decrease in PC, AOPP levels and normalized P-SH, T-SH, Np-SH groups in liver of diabetic animals. CONCLUSIONS: The results are in accordance with the pro-oxidant role of chronic hyperglycemia, and the ability of stobadine to attenuate protein oxidation and improving tissue reductive capacity may account, at least partly for its observed beneficial effects on tissue function in diabetes.
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Authors | Ahmet Cumaoglu, Cemal Cevik, Lucia Rackova, Nuray Ari, Cimen Karasu |
Journal | BioFactors (Oxford, England)
(Biofactors)
Vol. 30
Issue 3
Pg. 171-8
( 2007)
ISSN: 0951-6433 [Print] Netherlands |
PMID | 18525111
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Carbolines
- Sulfhydryl Compounds
- 3-nitrotyrosine
- Tyrosine
- Streptozocin
- dicarbine
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Carbolines
(pharmacology)
- Diabetes Mellitus, Experimental
(chemically induced, metabolism, prevention & control)
- Liver
(drug effects, metabolism)
- Male
- Oxidation-Reduction
(drug effects)
- Oxidative Stress
(drug effects)
- Protein Carbonylation
(drug effects)
- Rats
- Rats, Wistar
- Streptozocin
- Sulfhydryl Compounds
(metabolism)
- Tyrosine
(analogs & derivatives, metabolism)
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