Abstract | PURPOSE: METHODS: Wild-type and abca4(-/-) mice were fed normal or vitamin A-supplemented diets. Tissues from these mice were analyzed biochemically for retinoids and lipofuscin pigments. Eyes from these mice were analyzed morphologically for lipofuscin in the retinal pigment epithelium and for degeneration of photoreceptors. Visual function in these mice was analyzed by electroretinography. RESULTS: Mice that received vitamin A supplementation had dramatically higher levels of retinyl esters in the liver and retinal pigment epithelium. Lipofuscin pigments were significantly increased by biochemical and morphologic analysis in wild-type and abca4(-/-) mice fed the vitamin A-supplemented diet. Photoreceptor degeneration was observed in 11-month-old albino, but not pigmented, abca4(-/-) mice on both diets. CONCLUSIONS:
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Authors | Roxana A Radu, Quan Yuan, Jane Hu, Jennifer H Peng, Marcia Lloyd, Steven Nusinowitz, Dean Bok, Gabriel H Travis |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 49
Issue 9
Pg. 3821-9
(Sep 2008)
ISSN: 1552-5783 [Electronic] United States |
PMID | 18515570
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- ATP-Binding Cassette Transporters
- Abca4 protein, mouse
- Lipofuscin
- Vitamin A
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Topics |
- ATP-Binding Cassette Transporters
(genetics)
- Animals
- Dietary Supplements
- Disease Models, Animal
- Electroretinography
- Lipofuscin
(metabolism)
- Liver
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred Strains
- Mice, Knockout
- Pigment Epithelium of Eye
(metabolism)
- Retina
(pathology)
- Retinal Degeneration
(drug therapy, genetics)
- Spectrometry, Mass, Electrospray Ionization
- Vitamin A
(administration & dosage, therapeutic use)
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