Detection of primary Epstein-Barr virus infection in a patient with X-linked lymphoproliferative disease receiving immunoglobulin prophylaxis.

Abstract

Serologic diagnosis for Epstein-Barr virus (EBV) infection is problematic when patients receive exogenous immunoglobulin. We recently diagnosed primary EBV infection by detecting EBV-determined nuclear antigen (EBNA) and EBV-DNA in peripheral blood mononuclear cells (PBMC) using immunofluorescence, in situ hybridization and polymerase chain reaction (PCR) techniques in a patient with X-linked lymphoproliferative disease (XLP) who received prophylactic immunoglobulins for EBV infection. These combined techniques may be helpful for early and accurate diagnosis of EBV infection in highly vulnerable patients.

• Authors: M Okano, R M Bashir, J R Davis, D T Purtilo
• Journal: American journal of hematology (Am J Hematol) Vol. 36 Issue 4 Pg. 294-6 (Apr 1991) ISSN: 0361-8609 [Print] United States
• PMID: 1849350 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral
• DNA, Viral
• Epstein-Barr Virus Nuclear Antigens

Topics

• Adult
• Antigens, Viral (analysis)
• Base Sequence
• Burkitt Lymphoma (complications, diagnosis, prevention & control)
• DNA, Viral (analysis, genetics)
• Epstein-Barr Virus Nuclear Antigens
• Fluorescent Antibody Technique
• Herpesvirus 4, Human (genetics, immunology, isolation & purification)
• Humans
• Immunization, Passive
• Lymphoproliferative Disorders (complications, drug therapy, microbiology)
• Male
• Molecular Sequence Data
• Nucleic Acid Hybridization