Abstract |
Pirfenidone (PFD) is focused on a new anti-fibrotic drug, which can minimize lung fibrosis etc. We evaluated the superoxide (O2*-) scavenging activities of PFD and the PFD- iron complex by electron spin resonance (ESR) spectroscopy, luminol-dependent chemiluminescence assay, and cytochrome c reduction assay. Firstly, we confirmed that the PFD- iron complex was formed by mixing iron chloride with threefold molar PFD, and the complex was stable in distilled water and ethanol. Secondary, the PFD- iron complex reduced the amount of O2*- produced by xanthine oxidase/ hypoxanthine without inhibiting the enzyme activity. Thirdly, it also reduced the amount of O2*- released from phorbor ester-stimulated human neutrophils. PFD alone showed few such effects. These results suggest the possibility that the O2*- scavenging effect of the PFD- iron complex contributes to the anti-fibrotic action of PFD used for treating idiopathic pulmonary fibrosis.
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Authors | Yoshihiro Mitani, Keizo Sato, Yosuke Muramoto, Tomohiro Karakawa, Masataka Kitamado, Tatsuya Iwanaga, Tetsuji Nabeshima, Kumiko Maruyama, Kazuko Nakagawa, Kazuhiko Ishida, Kazumi Sasamoto |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 372
Issue 1
Pg. 19-23
(Jul 18 2008)
ISSN: 1090-2104 [Electronic] United States |
PMID | 18468515
(Publication Type: Journal Article)
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Chemical References |
- Free Radical Scavengers
- Pyridones
- Superoxides
- pirfenidone
- Iron
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Topics |
- Cell Line
- Free Radical Scavengers
(chemical synthesis, pharmacology, therapeutic use)
- Humans
- Iron
- Neutrophils
(drug effects, metabolism)
- Pulmonary Fibrosis
(drug therapy)
- Pyridones
(chemistry, pharmacology, therapeutic use)
- Superoxides
(chemistry, metabolism)
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