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A simple and improved method for diagnosis of tuberculosis using hypertonic saline and sodium hydroxide to concentrate and decontaminate sputum.

Abstract
We report the use of hypertonic saline and sodium hydroxide (HS-SH) for concentration-decontamination of sputum samples for the diagnosis of tuberculosis. This method increases detection of mycobacteria in smears compared to examination of direct smears, is comparable and reduces contamination in cultures compared to NALC-NaOH.
AuthorsJessica N Ricaldi, Humberto Guerra
JournalTropical doctor (Trop Doct) Vol. 38 Issue 2 Pg. 97-9 (Apr 2008) ISSN: 0049-4755 [Print] England
PMID18453499 (Publication Type: Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Saline Solution, Hypertonic
  • Sodium Hydroxide
Topics
  • Bacteriological Techniques
  • Decontamination (methods)
  • Humans
  • Mycobacterium tuberculosis (isolation & purification)
  • Saline Solution, Hypertonic (pharmacology)
  • Sodium Hydroxide (pharmacology)
  • Specimen Handling (methods)
  • Sputum (drug effects, microbiology)
  • Tuberculosis, Pulmonary (diagnosis, microbiology)

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