The aim of the present study was to evaluate the photodynamic efficacy of a novel
phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human
breast cancer cells and the LM2
adenocarcinoma subcutaneously implanted in Balb/c mice as experimental models. The S1-l-alpha-dimyristoyl-phosphatidylcholine
liposome was selected as the best delivery system because it showed greater internalization into cells (35 nmol/10(6) cells), relative to other
liposomes. After 3 h incubation S1 was partially localized in lysosomes, the compartment that represented its primary photodamage site. The S1 treated cultures also revealed a degree of mitochondrial morphology alteration. Indeed, S1 leads to photokilling of the cells with different efficacies indicating that cell photoinactivation was dependent on both the
phthalocyanine concentration and the light dose applied. Analyses of morphology and nuclear condensation level indicated that some of the cells exposed to
photodynamic therapy were undergoing apoptosis within 8h
after treatment. To assess the in vivo effectiveness of S1, animals bearing
tumors were treated with 0.2mg/kg S1 followed 24h later by 108 J cm(-2) light at 600-800 nm and 60 mW cm(-2),while other animals served as controls (no treatment, light alone, or S1 alone). All S1 treated
tumors and none of the controls exhibited complete or partial responses, and these responses continued for the entire observation period of 12 days. Evaluation of
tumor size showed that the treatment effectively delayed
tumor growth. Light microscopy investigations of irradiated
tumor specimens showed that S1 causes an early direct damage of malignant cells, largely via processes leading to random necrotic pathways.