HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An improved method to obtain antigen-excreting Toxocara canis larvae.

Abstract
Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781-782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol.
AuthorsNeuza Maria Alcântara-Neves, Alex Barbosa dos Santos, Lívia Ribeiro Mendonça, Camila A V Figueiredo, Lain Pontes-de-Carvalho
JournalExperimental parasitology (Exp Parasitol) Vol. 119 Issue 3 Pg. 349-51 (Jul 2008) ISSN: 1090-2449 [Electronic] United States
PMID18439580 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Helminth
Topics
  • Animals
  • Antigens, Helminth (metabolism)
  • Dog Diseases (parasitology)
  • Dogs
  • Feces (parasitology)
  • Female
  • Humans
  • Larva Migrans, Visceral (diagnosis)
  • Toxocara canis (immunology, isolation & purification)
  • Toxocariasis (parasitology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: