[Effect of pyrrolidine dithiocarbamate on antioxidation of canine myocardium during ichemia/reperfusion injury].

Abstract

OBJECTIVE:

To assess the alterations in myocardial energy metabolism and lipid peroxidation during canine cardiopulmonary bypass (CPB), and to investigate the interventional effects of pyrrolidine dithiocarbamate (PDTC) pretreatment.

METHODS:

Twelve adult healthy dogs undergoing CPB were randomized into control group (Group C, n=6) and PDTC group(Group P, n=6). In Group P, 30 mg/kg PDTC was administered intravenously before CPB and in Group C animals were given physiological saline instead of PDTC. The contents of adenosine triphosphate (ATP), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and mitochondrial swelling degree (MSD) of myocardium were determined before CPB, 60 min after aortic cross-clamping (AC) and 60 min after declamping (DC). Hemodynamics was monitored before CPB, 30 min and 60 min after DC.

RESULT:

Contents of ATP, SOD and GSH-PX in Group P at 60 min after AC and 60 min after DC were higher than those in Group C (P<0.01). MDA and MSD in Group P at 60 min after AC and 60 min after DC were significantly lower than those in Group C (P<0.01). Hemodynamics of Group P was recovered at 30 min and 60 min after DC.

CONCLUSION:

Pretreatment with PDTC is effective in improving antioxidation capacity of myocardium and ameliorates myocardial energy metabolism.

• Authors: Hua Fang, Chang-xi Li, Quan-yun Wang, Jin Liu, Yun-xia Zuo
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 37 Issue 2 Pg. 193-7 (03 2008) ISSN: 1008-9292 [Print] China
• PMID: 18422282 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antioxidants
• Pyrrolidines
• Thiocarbamates
• pyrrolidine dithiocarbamic acid
• Malondialdehyde
• Adenosine Triphosphate
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Antioxidants (pharmacology)
• Cardiopulmonary Bypass
• Dogs
• Female
• Glutathione Peroxidase (metabolism)
• Male
• Malondialdehyde (metabolism)
• Myocardial Reperfusion Injury (metabolism, prevention & control)
• Myocardium (metabolism)
• Pyrrolidines (pharmacology)
• Random Allocation
• Superoxide Dismutase (metabolism)
• Thiocarbamates (pharmacology)