Thrombospondin-1 expression correlates with angiogenesis in experimental cirrhosis.

Abstract

AIM:

To investigate the significance of Thrombospondin-1 (TSP-1) expression and its relationship with angiogenesis during experimental fibrosis.

METHODS:

Cirrhosis was induced in male Wistar rats by intraperitoneal administration of diethyl nitrosamine (DEN). The serial sections from liver tissues were stained with anti-CD34 and anti-TSP-1 antibodies before being quantitated by light microscopy.

RESULTS:

Our results showed that of TSP-1 expression gradually increases according to the severity of fibrosis (P < 0.05). Moreover, TSP-1 expression was found to be correlated with angiogenesis (P < 0.05).

CONCLUSION:

The correlative evidence of the link between TSP-1 and fibrosis or angiogenesis provided by this study suggests that besides its role as a strong promoter of transforming growth factor-beta 1 (TGF-beta 1), TSP-1 might have an additional role in liver fibrogenesis by stimulating angiogenesis and this protein could be a potential target to prevent fibrogenesis in chronic inflammatory diseases of the liver.

• Authors: Gulsum-Ozlem Elpek, Guzide-Ayse Gokhan, Sevgi Bozova
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 14 Issue 14 Pg. 2213-7 (Apr 14 2008) ISSN: 1007-9327 [Print] United States
• PMID: 18407596 (Publication Type: Journal Article)

Chemical References

• Antigens, CD34
• Thrombospondin 1
• Diethylnitrosamine

Topics

• Animals
• Antigens, CD34 (biosynthesis)
• Diethylnitrosamine (metabolism)
• Disease Models, Animal
• Fibrosis (metabolism)
• Gene Expression Regulation
• Immunohistochemistry (methods)
• Inflammation
• Liver (pathology)
• Male
• Neovascularization, Pathologic
• Rats
• Rats, Wistar
• Thrombospondin 1 (biosynthesis)