Abstract | OBJECTIVES: STUDY DESIGN: Randomized, blinded study using an in vivo rat model of microvenous failure. METHODS: Thirty-two rats received either desirudin or saline in a randomized, blinded fashion 30 minutes prior to performance of a standardized thrombogenic procedure on rat femoral veins. Bleeding time, vessel patency, and presence of clot within the anastomosis were subsequently assessed. Appropriate statistical analyses were then performed. RESULTS: There was a significant increase in vessel patency in rats treated preoperatively with desirudin compared to controls receiving saline (96.9% vs. 53.1%, P < .001). In evaluating patent vessels for non-occluding clot, 41.2% of control rats had non-obstructive clot at the site of anastomosis, versus 3.2% of rats treated with desirudin (P = .002). Bleeding times were longer in desirudin-treated rats than those that received saline (7.17 +/- 3 minutes vs. 5.15 +/- 1.2 minutes, P = .027). CONCLUSIONS: The use of preoperative desirudin increases the rate of microvascular anastomotic patency, decreases the occurrence of non-occluding clot, and increases bleeding time in an in vivo rat model, indicating potential efficacy in patients undergoing microvascular free tissue transfer.
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Authors | Carol M Lewis, Daniel G Deschler |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 118
Issue 7
Pg. 1149-52
(Jul 2008)
ISSN: 1531-4995 [Electronic] United States |
PMID | 18401270
(Publication Type: Journal Article)
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Chemical References |
- Fibrinolytic Agents
- Hirudins
- Recombinant Proteins
- desirudin
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Topics |
- Anastomosis, Surgical
- Animals
- Disease Models, Animal
- Fibrinolytic Agents
(pharmacology)
- Graft Survival
(drug effects)
- Hirudins
(pharmacology)
- Male
- Microcirculation
(drug effects, surgery)
- Microsurgery
- Preoperative Care
- Rats
- Rats, Sprague-Dawley
- Recombinant Proteins
(pharmacology)
- Surgical Flaps
(blood supply)
- Thrombosis
(prevention & control)
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