Site 1 protease is required for proteolytic processing of the glycoproteins of the South American hemorrhagic fever viruses Junin, Machupo, and Guanarito.

Abstract	The cellular proprotein convertase site 1 protease (S1P) has been implicated in the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. Here we report that S1P is also involved in the processing of the GPs of the genetically more-distant South American hemorrhagic fever viruses Guanarito, Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease recognition sites deviate from the reported S1P consensus sequence, indicates a broader specificity of S1P than anticipated. Lack of GP processing of Junin virus dramatically reduced production of infectious virus and prevented cell-to-cell propagation. Infection of S1P-deficient cells resulted in viral persistence over several weeks without the emergence of escape variants able to use other cellular proteases for GP processing.
Authors	Jillian M Rojek, Andrew M Lee, NgocThao Nguyen, Christina F Spiropoulou, Stefan Kunz
Journal	Journal of virology (J Virol) Vol. 82 Issue 12 Pg. 6045-51 (Jun 2008) ISSN: 1098-5514 [Electronic] United States
PMID	18400865 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	Glycoproteins Protein Precursors Recombinant Proteins Green Fluorescent Proteins Luciferases Proprotein Convertases Serine Endopeptidases membrane-bound transcription factor peptidase, site 1
Topics	Amino Acid Sequence Animals Arenaviruses, New World (metabolism) CHO Cells Cell Line Chlorocebus aethiops Cricetinae Cricetulus Glycoproteins (metabolism) Green Fluorescent Proteins (metabolism) Humans Kidney (cytology) Luciferases (analysis, metabolism) Molecular Sequence Data Mutation Proprotein Convertases (chemistry, genetics, metabolism) Protein Precursors (chemistry, metabolism) Protein Processing, Post-Translational Recombinant Proteins (chemistry, metabolism) Serine Endopeptidases (chemistry, genetics, metabolism) Transfection Vero Cells

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