Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression.

Abstract

The aim of the study was to investigate the influence of severe hyperthyroidism on plasma high-density lipoprotein cholesterol (HDL-C). Recently, it was shown in mice that increasing doses of T(3) up-regulate hepatic expression of scavenger receptor class B, type I, resulting in increased clearance of plasma HDL-C. Here, we show that severe hyperthyroidism in mice did not affect hepatic expression of scavenger receptor class B, type I, but reduced hepatic expression of ATP-binding cassette transporter 1, accompanied by a 40% reduction of HDL-C. The sterol content of bile, liver, and feces was markedly increased, accompanied by up-regulation of hepatic cholesterol 7alpha-hydroxylase, and ATP-binding cassette transporter 5, which is known to promote biliary sterol secretion upon dimerization with ATP-binding cassette transporter 8. Both control and hyperthyroid mice exerted identical plasma clearance of iv injected [(3)H]HDL-C, supporting the view that severe hyperthyroidism does not affect HDL-C clearance but, rather, its formation via hepatic ATP-binding cassette transporter 1.

• Authors: Ivan Tancevski, Andreas Wehinger, Egon Demetz, Philipp Eller, Kristina Duwensee, Julia Huber, Kathrin Hochegger, Wilfried Schgoer, Catherine Fievet, Frans Stellaard, Mats Rudling, Josef R Patsch, Andreas Ritsch
• Journal: Endocrinology (Endocrinology) Vol. 149 Issue 7 Pg. 3708-12 (Jul 2008) ISSN: 0013-7227 [Print] United States
• PMID: 18388200 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter 1
• ATP-Binding Cassette Transporters
• Cholesterol, HDL
• Cholesterol

Topics

• ATP Binding Cassette Transporter 1
• ATP-Binding Cassette Transporters (genetics, metabolism)
• Animals
• Bile (chemistry)
• Blotting, Western
• Cholesterol (metabolism)
• Cholesterol, HDL (blood)
• Feces (chemistry)
• Gene Expression
• Hyperthyroidism (blood, metabolism, physiopathology)
• Liver (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Reverse Transcriptase Polymerase Chain Reaction