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On the sulfation and methylation of catecholestrogens in human mammary epithelial cells and breast cancer cells.

Abstract
Prolonged exposure to high level of estrogen is a known risk factor for breast carcinogenesis. In cells, estrogens, in particular estrone (E1) and 17 beta-estradiol (E2), can be converted to catecholestrogens (CEs) which may be oxidized to form CE-semiquinones and CE-quinones that are capable of binding to DNA to induce mutations, followed by carcinogenesis. Whether the body is equipped with protective mechanisms against potentially harmful CEs, therefore, is an important issue. The present study was designed to examine the role of sulfation in the metabolism of CEs. MCF-7 breast cancer cells and MCF 10A human mammary epithelial cells were metabolically labeled with [35S]sulfate in the presence of individual CEs. Analysis of the labeling media showed the generation and release of exclusively [35S]sulfated 2-methoxy-E1 or [35S]sulfated 2- or 4-methoxy-E2 by cells labeled in the presence of 2-OH-E1 or 2- or 4-OH-E2. Whereas both [35S]sulfated 4-methoxy-E1 and [35S]sulfated 4-OH-E1 were detected in the labeling media of cells labeled in the presence of 4-OH-E1. These results indicated a concerted action of catechol-O-methyltransferase (COMT) and the cytosolic sulfotransferase (SULT) enzyme(s) in the metabolism of CEs. Enzymatic assays revealed that, five (SULT1A1, SULT1A2, SULT1A3, SULT1C4, and SULT1E1) of eleven known human SULTs tested could use CEs and methoxyestrogens (MEs) as substrates, with SULT1E1 displaying the strongest sulfating activity.
AuthorsYing Hui, Shin Yasuda, Ming-Yih Liu, Yi-Yong Wu, Ming-Cheh Liu
JournalBiological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 31 Issue 4 Pg. 769-73 (Apr 2008) ISSN: 0918-6158 [Print] Japan
PMID18379081 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Catechols
  • Estrogens
  • Recombinant Proteins
  • Sulfates
  • Sulfur Radioisotopes
  • Tropolone
  • Catechol O-Methyltransferase
  • Sulfotransferases
Topics
  • Breast (cytology, metabolism)
  • Breast Neoplasms (metabolism)
  • Catechol O-Methyltransferase (metabolism)
  • Catechols (chemistry)
  • Cell Line, Tumor
  • Cytosol (enzymology, metabolism)
  • Epithelial Cells (metabolism)
  • Estrogens (chemistry)
  • Female
  • Humans
  • Methylation
  • Recombinant Proteins (chemistry)
  • Sulfates (metabolism)
  • Sulfotransferases (metabolism)
  • Sulfur Radioisotopes (chemistry)
  • Tropolone (pharmacology)

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