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High speed gradient elution reversed phase liquid chromatography of bases in buffered eluents. Part II. Full equilibrium.

Abstract
In this work we determined when the state of thermodynamic (full) equilibrium, i.e. time-invariate solute retention, was achieved in gradient elution reversed-phase chromatography. We investigated the effects of flow rate, temperature, organic modifier, buffer type/concentration, stationary phase type, n-butanol as eluent additive, and pore size. We also measured how selectivity varied with reequilibration time. Stationary phase wetting and the ability of the stationary phase to resist changes in pH strongly affect the time needed to reach full equilibrium. For example, full equilibrium is realized with many endcapped stationary phases after flushing with only two column volumes of acetonitrile-water containing 1% (v/v) n-butanol and 0.1% (v/v) trifluoroacetic acid. Trends in retention time (<0.010min) and selectivity become quite small after only five column volumes of reequilibration. We give practical guidelines that provide fast full equilibrium for basic compounds when chromatographed in buffered eluents.
AuthorsAdam P Schellinger, Dwight R Stoll, Peter W Carr
JournalJournal of chromatography. A (J Chromatogr A) Vol. 1192 Issue 1 Pg. 54-61 (May 23 2008) ISSN: 0021-9673 [Print] Netherlands
PMID18374933 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Buffers
  • Peptide Fragments
  • Pharmaceutical Preparations
  • Serum Albumin, Bovine
  • 1-Butanol
Topics
  • 1-Butanol (chemistry)
  • Buffers
  • Chromatography, Liquid (methods)
  • Hydrogen-Ion Concentration
  • Peptide Fragments (isolation & purification)
  • Pharmaceutical Preparations (isolation & purification)
  • Porosity
  • Serum Albumin, Bovine (chemistry)
  • Temperature

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