Abstract |
We investigated the effect of neuropeptides, which are vasoactive intestinal polypeptide (VIP), substance P, (SP), neuropeptide Y (NPY), neurokinin A (NKA), somatostatin (SOM), calcitonin gene-related peptide (CGRP), and leucine-enkephalin (L-ENK), on the invasion of murine Colon 26-L5 adenocarcinoma cells through a reconstituted basement membrane ( Matrigel) using a Transwell cell culture chamber assay. VIP, SP, NPY, and L-ENK reduced invasive potential of tumor cells in a concentration-dependent manner, whereas SOM, CGRP, and NKA had no effect. Especially, VIP showed the most effective in inhibiting tumor invasion, and achieved 50% reduction at 10(-6) M. A similar effect by VIP was also observed in cell migration to fibronectin. VIP had no effect on the growth of tumor cells at the concentrations ranging from 10(-10) to 10(-6) M. The suppressed ability of the tumor cell motility by VIP (10(-6) M) was practically recovered by co-treatment with 2',5'-dideoxyadenosine, an adenylate cyclase inhibitor. These results indicate that VIP, among the neuropeptides used, could inhibit Matrigel invasion of Colon 26-L5 carcinoma cells through partial suppression of their motility, and the reduction was associated with an intracellular cAMP-mediated pathway.
|
Authors | M Ogasawara, J Murata, K Ayukawa, I Saiki |
Journal | Cancer letters
(Cancer Lett)
Vol. 119
Issue 1
Pg. 125-30
(Oct 28 1997)
ISSN: 0304-3835 [Print] Ireland |
PMID | 18372531
(Publication Type: Corrected and Republished Article, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Adenocarcinoma
(metabolism, pathology)
- Animals
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Colonic Neoplasms
(metabolism, pathology)
- In Vitro Techniques
- Mice
- Neoplasm Invasiveness
- Neuropeptides
(pharmacology)
|