[Induction of insulin resistance induced by PI-3K inhibitor in porcine granulosa cells].

Abstract

OBJECTIVE:

To investigate the biological effects of insulin resistance (IR) on the porcine granulosa cells which is induced by wortmannin, the PI-3K inhibitor and mediated by key molecules including GLUT4 and MAPK during insulin signaling.

METHODS:

The model of IR porcine granulosa cell was established in in vitro culture by treatment of wortmannin, and was assessed the amount of 3H glucose uptake as well as medium glucose levels by glucose oxidase method. The protein and mRNA expression of GLUT4 and MAPK were evaluated by immunofluorescence and RT-PCR respectively.

RESULTS:

The glucose intake was decreased by 40% with treatment of wortmannin at 1.5 micromol/L (P<0.05). GLUT4 and MAPK were localized mainly to cytoplasm of granulose cells. When granulosa cells were insulin resistant, the expression of GLUT4 was down-regulated whereas MAPK was up-regulated as compared with the controls.

CONCLUSIONS:

Wortmannin treatment can lead to decreased expression of GLUT4 and increase of IR granulose cells. This metabolic phenotype could induce increased expression of MAPK and mitogenic potential, indicating the cross-talk between two pathways of insulin signaling within ovarian cells.

• Authors: Miao-e Yan, Xiao-ke Wu, Juan-juan Song, Li-hui Hou
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 43 Issue 1 Pg. 54-8 (Jan 2008) ISSN: 0529-567X [Print] China
• PMID: 18366935 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Androstadienes
• Glucose Transporter Type 4
• Phosphoinositide-3 Kinase Inhibitors
• Protein Kinase Inhibitors
• Mitogen-Activated Protein Kinases
• Glucose
• Wortmannin

Topics

• Androstadienes (administration & dosage, pharmacology)
• Animals
• Cells, Cultured
• Disease Models, Animal
• Female
• Fluorescent Antibody Technique
• Glucose (metabolism, pharmacokinetics)
• Glucose Transporter Type 4 (genetics, metabolism)
• Granulosa Cells (cytology, drug effects, metabolism)
• Insulin Resistance
• Mitogen-Activated Protein Kinases (genetics, metabolism)
• Ovary (cytology)
• Phosphoinositide-3 Kinase Inhibitors
• Protein Kinase Inhibitors (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction (drug effects)
• Swine
• Wortmannin