Abstract |
The Sgt1 protein is a binding partner of heat shock proteins such as Hsp90, Hsp70 or Hsc70. In this work we show that the level of Sgt1 is increased in HEp-2 cells exposed to heat shock or radicicol. The citrate synthase aggregation assay shows that Sgt1 attenuates aggregation of the enzyme induced by increased temperature as efficiently as p23, a known co-chaperone of Hsp90. We have cloned two fragments of the human Sgt1 gene promoter (-708/+98 and -351/+98) into pGL3-luciferase vector and found that both fragments generated a 2-fold increase in luciferase activity upon heat shock. Furthermore, electrophoretic mobility shift assay revealed binding of the HSF-1 transcription factor to the heat shock element in the proximal (-42/-2) Sgt1 gene promoter fragment. These results indicate that Sgt1 is a co-chaperone protein with an expression pattern matching that of the well known heat shock proteins.
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Authors | Magdalena Zabka, Wiesława Leśniak, Wiktor Prus, Jacek Kuźnicki, Anna Filipek |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 370
Issue 1
Pg. 179-83
(May 23 2008)
ISSN: 1090-2104 [Electronic] United States |
PMID | 18358234
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Cycle Proteins
- DNA-Binding Proteins
- Heat Shock Transcription Factors
- Macrolides
- Molecular Chaperones
- Protein Kinase Inhibitors
- SUGT1 protein, human
- Transcription Factors
- Luciferases
- Citrate (si)-Synthase
- monorden
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Topics |
- Animals
- Cell Cycle Proteins
(chemistry, genetics, metabolism)
- Cell Line
- Cells, Cultured
- Citrate (si)-Synthase
(chemistry)
- DNA-Binding Proteins
(metabolism)
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation
- Genes, Reporter
- Heat Shock Transcription Factors
- Heat-Shock Response
- Humans
- Luciferases
(genetics)
- Macrolides
(pharmacology)
- Molecular Chaperones
(chemistry, genetics, metabolism)
- Promoter Regions, Genetic
- Protein Kinase Inhibitors
(pharmacology)
- Transcription Factors
(metabolism)
- Up-Regulation
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