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Sgt1 has co-chaperone properties and is up-regulated by heat shock.

Abstract
The Sgt1 protein is a binding partner of heat shock proteins such as Hsp90, Hsp70 or Hsc70. In this work we show that the level of Sgt1 is increased in HEp-2 cells exposed to heat shock or radicicol. The citrate synthase aggregation assay shows that Sgt1 attenuates aggregation of the enzyme induced by increased temperature as efficiently as p23, a known co-chaperone of Hsp90. We have cloned two fragments of the human Sgt1 gene promoter (-708/+98 and -351/+98) into pGL3-luciferase vector and found that both fragments generated a 2-fold increase in luciferase activity upon heat shock. Furthermore, electrophoretic mobility shift assay revealed binding of the HSF-1 transcription factor to the heat shock element in the proximal (-42/-2) Sgt1 gene promoter fragment. These results indicate that Sgt1 is a co-chaperone protein with an expression pattern matching that of the well known heat shock proteins.
AuthorsMagdalena Zabka, Wiesława Leśniak, Wiktor Prus, Jacek Kuźnicki, Anna Filipek
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 370 Issue 1 Pg. 179-83 (May 23 2008) ISSN: 1090-2104 [Electronic] United States
PMID18358234 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Heat Shock Transcription Factors
  • Macrolides
  • Molecular Chaperones
  • Protein Kinase Inhibitors
  • SUGT1 protein, human
  • Transcription Factors
  • Luciferases
  • Citrate (si)-Synthase
  • monorden
Topics
  • Animals
  • Cell Cycle Proteins (chemistry, genetics, metabolism)
  • Cell Line
  • Cells, Cultured
  • Citrate (si)-Synthase (chemistry)
  • DNA-Binding Proteins (metabolism)
  • Electrophoretic Mobility Shift Assay
  • Gene Expression Regulation
  • Genes, Reporter
  • Heat Shock Transcription Factors
  • Heat-Shock Response
  • Humans
  • Luciferases (genetics)
  • Macrolides (pharmacology)
  • Molecular Chaperones (chemistry, genetics, metabolism)
  • Promoter Regions, Genetic
  • Protein Kinase Inhibitors (pharmacology)
  • Transcription Factors (metabolism)
  • Up-Regulation

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