[Inhibitory effect of anti-Lewis y antibody on alpha1,2-fucosyltransferase gene transfected human ovarian cancer cells in vitro].

Abstract

AIM:

To study the inhibitory effect of anti-Lewis y monoclonal antibody on proliferation, adhesion and invasion of alpha1, 2-fucosyltransferase gene transfected human ovarian cancer cell line RMG-I-H with over-expression of Lewis y antigen in vitro.

METHODS:

The changes of proliferation, adhesion and invasion of RMG-I-H cells in vitro were detected by cell growth assay, cell adhesive assay and cell invasive assay, respectively, with goat anti-human IgG antibody treated group or non-antibody treated group as control.

RESULTS:

The proliferation and adhesion of RMG-I-H cells were remarkably inhibited by preincubation of the tumor cells with anti-Lewis y antibody in vitro (P<0.05). The growth inhibitory rate from the second day to the seventh day was 5.62%, 19.75%, 34.96%, 46.51%, 49.78% and 33.33%, respectively. The adhesion inhibitory rate at different time points (15, 30, 60 min) was 47.59%, 58.64% and 13.85%, respectively. Invasive assay with a transwell cell culture chamber showed that there were no significant differences of invasion in vitro between each concentration of the Lewis y antibody treated group and non-antibody treated group (P>0.05).

CONCLUSION:

Anti-Lewis y antibody significantly inhibits the proliferation and adhesion of RMG-I-H cells cultured in vitro, but it has no effect on invasion, which indicates that Lewis y is associated with some biological behaviors, such as proliferation and adhesion. The antibody against Lewis y may be an effective anti-metastasis agent for the treatment of patients with ovarian cancer.

• Authors: Fei-fei Li, Bei Lin, Ying-ying Hao, Juan-juan Liu, Fan Zhang, Shu-lan Zhang
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 24 Issue 3 Pg. 267-9 (Mar 2008) ISSN: 1007-8738 [Print] China
• PMID: 18328190 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Lewis Blood Group Antigens
• Lewis Y antigen
• Fucosyltransferases

Topics

• Antibodies, Monoclonal (immunology, pharmacology, therapeutic use)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Female
• Fucosyltransferases (genetics, physiology)
• Humans
• Lewis Blood Group Antigens (immunology)
• Ovarian Neoplasms (drug therapy)
• Transfection