Abstract |
The outbreak of H5N1 avian influenza strains infectious to human has dire neurological and pathological consequences. This led to the massive vaccination of host poultry, resulting in a Fujian-like variant (vFJ) resistant to immunization with two mutations at the furin-processing site of hemagglutinin: loss of the P2 Lys and P9 substitution of Gln to Leu within the cleavage site. We synthesized 14mer peptides mimicking the processing site of Fujian-like strains. We found that the peptide with the vFJ sequence is less cleaved as compared to the parent FJ-derived peptide by furin at either neutral or acidic pH values. We hypothesize that the double hemagglutinin mutations in vFJ may result in viruses with less processed hemagglutinin, thereby providing a mechanism for evading immune neutralization.
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Authors | Antonella Pasquato, Nabil G Seidah |
Journal | Journal of molecular neuroscience : MN
(J Mol Neurosci)
Vol. 35
Issue 3
Pg. 339-43
(Jul 2008)
ISSN: 0895-8696 [Print] United States |
PMID | 18306058
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemagglutinins, Viral
- Influenza Vaccines
- Peptides
- Heparin
- Furin
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Topics |
- Amino Acid Substitution
(genetics)
- Animals
- Binding Sites
(genetics)
- Binding, Competitive
(genetics)
- Birds
- Evolution, Molecular
- Furin
(chemistry, metabolism)
- Genetic Variation
- Hemagglutinins, Viral
(chemistry, genetics, metabolism)
- Heparin
(chemistry)
- Humans
- Influenza A Virus, H5N1 Subtype
(genetics, immunology, metabolism, pathogenicity)
- Influenza Vaccines
(chemistry)
- Influenza in Birds
(genetics, prevention & control)
- Influenza, Human
(genetics)
- Mutation
(genetics)
- Peptides
(chemistry, genetics, metabolism)
- Vaccination
(adverse effects)
- Virulence
(genetics)
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