Abstract | BACKGROUND: METHODS: Rats received a single intravenous injection of OxPAPC (1.5 mg/kg) followed by mechanical ventilation at low tidal volume (LTV) (7 mL/kg) or HTV (20 mL/kg). Bronchoalveolar lavage was performed and lung tissue was stained for histological analysis. In vitro, the effects of OxPAPC on endothelial barrier dysfunction and GTPase activation were assessed in cells exposed to thrombin and pathologic (18%) cyclic stretch. RESULTS: HTV induced profound increases in bronchoalveolar lavage and tissue neutrophils and in lavage protein. Intravenous OxPAPC markedly attenuated HTV-induced protein and inflammatory cell accumulation in bronchoalveolar lavage fluid and lung tissue. In vitro, high-magnitude stretch enhanced thrombin-induced endothelial paracellular gap formation associated with Rho activation. These effects were dramatically attenuated by OxPAPC and were associated with OxPAPC-induced activation of Rac. CONCLUSION:
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Authors | Stephanie Nonas, Anna A Birukova, Panfeng Fu, Jungjie Xing, Santipongse Chatchavalvanich, Valery N Bochkov, Norbert Leitinger, Joe G N Garcia, Konstantin G Birukov |
Journal | Critical care (London, England)
(Crit Care)
Vol. 12
Issue 1
Pg. R27
( 2008)
ISSN: 1466-609X [Electronic] England |
PMID | 18304335
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Phosphatidylcholines
- Phospholipids
- oxidized-L-alpha-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine
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Topics |
- Animals
- Bronchoalveolar Lavage Fluid
(cytology)
- Cytokines
(biosynthesis)
- Electric Impedance
- Male
- Phosphatidylcholines
(therapeutic use)
- Phospholipids
(metabolism)
- Pneumonia
(metabolism, pathology, prevention & control)
- Rats
- Rats, Inbred BN
- Respiration, Artificial
(adverse effects)
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