[Impact of up-regulating Ezrin expression by Epstein-Barr virus latent membrane protein 1 on metastasis ability of nasopharyngeal carcinoma cells].

Abstract

BACKGROUND & OBJECTIVE:

Ezrin, a cytoskeleton-related protein, contributes to the invasion and metastasis of tumors; our previous study had also shown that Epstein-Barr virus latent membrane protein 1 (EBV-LMP1) can promote the metastasis of nasopharyngeal carcinoma (NPC) cells. This study was to investigate whether EBV-LMP1 could change the expression of Ezrin and the role of Ezrin on the metastasis of NPC cells.

METHODS:

The expression of EBV-LMP1 and Ezrin in NPC cell lines CNE1 (well differentiated NPC cell line) and CNE1-GL (CNE1 cells stably transfected with EBV-LMP1) were detected by SP immunohistochemistry and Western blot. The adhesive abilities of CNE1, CNE1-GL and AntiEzrin-CNE1-GL (CNE1-GL cells pretreated with Ezrin antibody) cells were assessed by cell-matrix adhesion assay. The invasion and migration of these cell lines were investigated by Transwell method.

RESULTS:

Ezrin was not expressed in CNE1 cells while strongly expressed in CNE1-GL cells. The adhesive ability of CNE1-GL cells with matrix was significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells [adhesive rate: (89.38+/-6.12)% vs. (49.42+/-5.37)% and (56.94+/-4.08)%, P<0.001, P<0.05]. The invasion and migration of CNE1-GL cells were significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells (invasive cells: 107+/-11 vs. 27+/-3 and 38+/-4, P<0.001; migratory cells: 179+/-25 vs. 46+/-6 and 51+/-5, P<0.001).

CONCLUSION:

EBV-LMP1 contributes to the metastasis ability of CNE1 cells by up-regulating the expression of Ezrin.

• Authors: Zhi-Hua Shen, Xiao-Yi Chen, Jin Chen
• Journal: Ai zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 27 Issue 2 Pg. 165-9 (Feb 2008) China
• PMID: 18279614 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytoskeletal Proteins
• EBV-associated membrane antigen, Epstein-Barr virus
• Viral Matrix Proteins
• ezrin

Topics

• Cell Line, Tumor
• Cell Movement
• Cytoskeletal Proteins (genetics)
• Humans
• Nasopharyngeal Neoplasms (pathology)
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Up-Regulation
• Viral Matrix Proteins (physiology)