Abstract |
Glutamate toxicity has been implicated in various retinal diseases. Green tea leaf extract catechin has protective effects against cellular toxicity. This study investigated the effects of catechin on the glutamate-treated retina. Porcine retinal homogenates were incubated with glutamate (20 nmol) at 37 degrees C for 60 min. Catechin was co-incubated with the glutamate-treated retina in the same condition. The malondialdehyde (MDA) levels were determined as an index of lipid peroxidation (LPO). Differential protein expressions were derived from two-dimensional gel electrophoresis. Mass spectrometry was conducted to identify the proteins. Glutamate increased the retinal MDA (p<0.0001) and catechin reversed the effect (p<0.0001). There were significant changes in seven proteins after the glutamate treatment (p<0.05), namely, heterogeneous ribonucleoprotein, thioredoxin peroxidase, 5-hydroxytryptamine receptor, pyruvate dehydrogenase, ARHA protein, peroxiredoxin 6 and proteasome. Catechin significantly reversed the changes in thioredoxin peroxidase, 5-hydroxytryptamine receptor, peroxiredoxin 6 and pyruvate dehydrogenase (p<0.05). Our study shows that (a) retinal glutamate toxicity is mediated by LPO and protein modification, and (b) catechin ameliorates the toxicity.
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Authors | Andrew W Siu, Mick K Lau, John S Cheng, Catherine K Chow, Wai C Tam, K K Li, Daniel K L Lee, Tony S S To, Chi H To, Chi W Do |
Journal | Neuroscience letters
(Neurosci Lett)
Vol. 432
Issue 3
Pg. 193-7
(Feb 27 2008)
ISSN: 0304-3940 [Print] Ireland |
PMID | 18249068
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Proteins
- Glutamic Acid
- Malondialdehyde
- Catechin
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Topics |
- Animals
- Catechin
(pharmacology)
- Dose-Response Relationship, Drug
- Drug Interactions
- Electrophoresis, Gel, Two-Dimensional
(methods)
- Glutamic Acid
(toxicity)
- In Vitro Techniques
- Lipid Peroxidation
(drug effects)
- Malondialdehyde
(metabolism)
- Mass Spectrometry
(methods)
- Proteins
(metabolism)
- Retina
(drug effects)
- Swine
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