[Detection of herpesvirus genome by multiplex polymerase chain reaction (PCR) and real-time PCR in ocular fluids of patients with acute retinal necrosis].

Abstract

PURPOSE:

The human herpesvirus (HHV) family consists of types 1 to 8 (HHV1-8). The purpose of this study was to investigate the detection of HHV DNA, especially HSV1 (herpes simplex virus 1, HHV1), HSV2 (herpes simplex virus 2, HHV2), and VZV (varicella-zoster virus, HHV3) in ocular fluids of patients with acute retinal necrosis(ARN).

METHODS:

The intraocular genome for HHV1-8 was determined in 19 ocular fluid samples (12 vitreous fluid and 7 aqueous humor samples) taken from ARN patients (n=14). The samples were tested for the presence of virus DNA by two systems of polymerase chain reaction (PCR): the multiplex PCR screening test and real-time quantitative PCR.

RESULTS:

Multiplex PCR demonstrated VZV (n=16, 84%), HSV1 (n = 1.5%) or HSV2 (n = 2.11%)genomic DNA in all the samples. In real-time PCR, a high copy number of virus DNA was detected. The virus DNA-positive samples contained Epstein-Barr virus (EBV, HHV4) DNA in 9 of 19 samples (47%). No HHV6-8 DNA was detected in the ocular samples, and no virus DNA was detected in the serum samples.

CONCLUSIONS:

The genome for HHV1-3 was detected in the patients with ARN. All cases contained a high copy number for the virus DNA that indicates viral replication. PCR systems are useful for determing whether virus infections are associated with uveitis.

• Authors: Sunao Sugita, Youichi Iwanaga, Tatsushi Kawaguchi, Yuri Futagami, Shintaro Horie, Tomoka Usui, Sayaka Yamamoto, Yoshiharu Sugamoto, Manabu Mochizuki, Norio Shimizu, Ken Watanabe, Miki Mizukami, Tomohiro Morio
• Journal: Nippon Ganka Gakkai zasshi (Nippon Ganka Gakkai Zasshi) Vol. 112 Issue 1 Pg. 30-8 (Jan 2008) ISSN: 0029-0203 [Print] Japan
• PMID: 18240601 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA, Viral

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Aqueous Humor (virology)
• DNA, Viral (analysis)
• Female
• Genome, Viral
• Herpesviridae (genetics, isolation & purification)
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction (methods)
• Retinal Necrosis Syndrome, Acute (virology)