Amelioration of anti-cancer agent adriamycin-induced nephrotic syndrome in rats by Wulingsan (Gorei-San), a blended traditional Chinese herbal medicine.

Anti-cancer agent adriamycin (ADR) has demonstrated high anti-tumor efficacy. However, its use in chemotherapy has been limited largely due to its diverse toxicities, including renal toxicity, such as nephrotic syndrome with proteinuria. Podocyte injury leads to glomeruli proteinuria. Wulingsan (WLS) is a blended traditional Chinese herbal medicine specifically used for various kidney diseases. In the present study, we found that a water extract of WLS (480 mg/kg, p.o., x 28 days) reduced ADR-induced increase in urine protein excretion, plasma total cholesterol and triglyceride, and decrease in plasma total protein and albumin in rats. Furthermore, the results of electron microscopy demonstrated suppression by WLS of ADR-induced increase in width of foot process, increase in surface density and decrease in volume density. These results suggest that WLS ameliorates ADR-induced proteinuria and podocyte injury. Gene analysis results demonstrated a suppression of renal overexpression of nephrin mRNA and protein by WLS. Radioimmunoassay showed that WLS suppressed ADR-induced increased renal angiotensin II content in rats. Thus our results demonstrate that WLS ameliorates ADR-induced nephrotic syndrome in rats possibly by suppressing ADR-induced hyperactivity of renal renin-angiotensin system to modulate renal nephrin gene expression, thereby protecting podocyte from injury.
AuthorsLan He, Xianglu Rong, Jian Min Jiang, Pei Qing Liu, Yuhao Li
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 46 Issue 5 Pg. 1452-60 (May 2008) ISSN: 0278-6915 [Print] England
PMID18215452 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibiotics, Antineoplastic
  • Membrane Proteins
  • Plant Extracts
  • RNA, Messenger
  • nephrin
  • wulingsan
  • Angiotensin II
  • Uric Acid
  • Doxorubicin
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Angiotensin II (metabolism)
  • Animals
  • Antibiotics, Antineoplastic (antagonists & inhibitors, toxicity)
  • Blotting, Western
  • Doxorubicin (antagonists & inhibitors, toxicity)
  • Glyceraldehyde-3-Phosphate Dehydrogenases (metabolism)
  • Kidney (drug effects, metabolism, pathology)
  • Male
  • Membrane Proteins (biosynthesis, genetics)
  • Microscopy, Electron, Transmission
  • Nephrotic Syndrome (chemically induced, pathology, prevention & control)
  • Plant Extracts (therapeutic use)
  • Podocytes (pathology, ultrastructure)
  • RNA, Messenger (biosynthesis, genetics)
  • Rats
  • Rats, Sprague-Dawley
  • Uric Acid (urine)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: