Effects of ammonium tetrathiomolybdate, an oncolytic/angiolytic drug on the viability and proliferation of endothelial and tumor cells.

Abstract

OBJECTIVE:

This study examines the target specificity of tetrathiomolybdate (ATM), an anti-angiogenic, anti-tumor agent against the viability / proliferation of arterial, venous, capillary endothelial and tumor cells.

MATERIALS AND METHODS:

Cells were seeded at 10 or 100% density (to measure viability / proliferation respectively) in medium +/- 0-250 uM ATM. Viability and proliferation were measured by metabolic labeling-colorimetry. One-way ANOVA + Bonferroni testing examined base metabolism; Dunnett's testing was used for viability/proliferation.

RESULTS:

Venous proliferation showed high ATM sensitivity (50% reduction ~ > or =5 uM ATM, p<0.01), capillary proliferation was inhibited > or =10 uM (p<0.05). Arterial endothelium were less sensitive to ATM, (50% inhibition ~ > or = 20 uM, p<0.01). YPEN-1 were inhibited >50 uM ATM. Capillary viability was inhibited > or =20 microM ATM (p<0.01); venous, arterial and tumor viability show less ATM sensitivity.

CONCLUSIONS:

Our data suggest that venous and capillary endothelial proliferation are important targets in ATM therapy, but that other vascular segments and tumor cells may be less influenced.

• Authors: A Carpenter, A Rassam, M H Jennings, S Robinson-Jackson, J S Alexander, C Erkuran-Yilmaz
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 56 Issue 12 Pg. 515-9 (Dec 2007) ISSN: 1023-3830 [Print] Switzerland
• PMID: 18210236 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inhibitors
• Molybdenum
• tetrathiomolybdate

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Endothelial Cells (cytology, drug effects)
• Endothelium, Vascular (cytology)
• Humans
• Molybdenum (pharmacology)
• Rats