Abstract | INTRODUCTION: AIM: METHODS: MAIN OUTCOME MEASURES: RESULTS: Maximum ICP (MaxICP) and ICP area under the curve, which were normalized by MAP, were significantly reduced in Ang II-infused hypertensive rats compared to that of normotensive rats (P < 0.05). Protein expression of NADPH oxidase subunit p47( phox) was significantly increased by 30% in Ang II-infused hypertensive rat penes along with increased DHE staining and TBARS levels (P < 0.05) when compared to that of controls. There were no significant changes in p67( phox) or gp91(phox) protein expression. Apocynin reduced NADPH oxidase protein expression and TBARS levels as well as improved MaxICP and ICP area under curve in Ang II-infused hypertensive rats (P < 0.05). CONCLUSIONS: These data suggest that activation of NADPH oxidase is a molecular mechanism for hypertension-associated ED. Apocynin treatment exerted protective effects on erectile function through inhibition of NADPH oxidase activity, thereby reducing oxidative stress in Ang II-infused hypertensive rats. This is the first study to identify the importance of NADPH oxidase in the regulation of erectile function in vivo.
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Authors | Liming Jin, Gwen Lagoda, Romulo Leite, R Clinton Webb, Arthur L Burnett |
Journal | The journal of sexual medicine
(J Sex Med)
Vol. 5
Issue 3
Pg. 544-51
(Mar 2008)
ISSN: 1743-6109 [Electronic] Netherlands |
PMID | 18208505
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Blood Pressure
(physiology)
- Disease Models, Animal
- Down-Regulation
- Enzyme Activation
- Erectile Dysfunction
(enzymology, etiology)
- Hypertension
(complications, enzymology)
- Male
- Muscle, Smooth, Vascular
(enzymology)
- NADPH Oxidases
(metabolism)
- Rats
- Rats, Sprague-Dawley
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