Prospective clinical and experimental studies on the cardioprotective effect of ulinastatin following severe burns.

Abstract

OBJECTIVE:

To investigate the preventive effect of ulinastatin on shock in the heart after burn.

METHODS:

In an open prospective clinical study 34 adults with burns >50% total body surface area were randomly divided into control (B) and ulinastatin-treated (U) groups. All underwent routine treatment, and group U received 100,000U ulinastatin intravenously three times a day for 1 week. In an animal experiment, 72 healthy rats underwent equivalent burn, similar division into groups B and U, and resuscitation according to Parkland's formula. Rats in group U received ulinastatin (40,000U/kg) immediately after burn. Myocardial pathomorphology, plasma cTnI, CK-MB and PMNE, myocardial MDA, TNF-alpha, IL-10 and caspase-3 activity and cardiocyte apoptosis were determined.

RESULTS:

Plasma cTnI, CK-MB, and PMNE were higher in clinical group B than group U. In the animal experiment, plasma cTnI, CK-MB, myocardial MDA, TNF-alpha, IL-10 and caspase-3 activity, and apoptotic index and myocardial pathomorphological changes were significantly less in group U than in group B, save IL-10.

CONCLUSION:

The clinical and experimental data showed that ulinastatin relieved myocardial damage from severe burn. The mechanism might involve modulation of the anti- and pro-inflammatory balance and lipid peroxidation, and decreased myocardiocyte apoptosis.

• Authors: Yuesheng Huang, Kang Xie, Jiaping Zhang, Yongming Dang, Zhang Qiong
• Journal: Burns : journal of the International Society for Burn Injuries (Burns) Vol. 34 Issue 5 Pg. 674-80 (Aug 2008) ISSN: 0305-4179 [Print] Netherlands
• PMID: 18201827 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Glycoproteins
• Inflammation Mediators
• Troponin I
• urinastatin

Topics

• Adult
• Animals
• Apoptosis (drug effects)
• Burns (blood, complications, pathology)
• Cardiotonic Agents (administration & dosage, therapeutic use)
• Disease Models, Animal
• Drug Administration Schedule
• Drug Evaluation, Preclinical
• Female
• Glycoproteins (administration & dosage, therapeutic use)
• Humans
• Inflammation Mediators (blood)
• Male
• Middle Aged
• Myocardial Ischemia (blood, etiology, pathology, prevention & control)
• Prospective Studies
• Rats
• Rats, Sprague-Dawley
• Shock, Cardiogenic (blood, etiology, pathology, prevention & control)
• Troponin I (blood)
• Young Adult