Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation.

Abstract

OBJECTIVE:

To evaluate the effects of amlodipine as an antioxidant and analyze the histopathologic changes in experimental ischemic and ischemic-reperfusion (I/R) injury in rat ovaries.

DESIGN:

Experimental study.

SETTING:

Experimental surgery laboratory.

ANIMAL(S):

Forty-two rats with experimentally induced ovarian torsion.

INTERVENTION(S):

Group 1: sham operation; group 2: bilateral ovarian ischemia; group 3: 3-hour period of ischemia plus 3 hours of reperfusion; groups 4 and 5: amlodipine administration at 3 and 5 mg/kg respectively before one half hour of ischemia, and then bilateral ovarian ischemia. The ovaries were removed at the third hour of ischemia. Groups 6 and 7: 3-hour period of bilateral ovarian ischemia. Two and a half hours after the induction of ischemia, the rats received amlodipine. At the end of a 3-hour period of ischemia, 3 hours of reperfusion was continued; then the ovaries were removed.

MAIN OUTCOME MEASURE(S):

Ovarian tissue superoxide dismutase and nitric oxide activity; histopathologic examination of all ovarian rat tissue.

RESULT(S):

Ischemia and I/R increased the inducible nitric oxide synthase activity while decreasing the superoxide dismutase activity significantly in comparison with the sham group. Both doses of amlodipine before ischemia and I/R reversed the trend in nitric oxide synthase activities and reversed the trend in the rat's ovary.

CONCLUSION(S):

Conservative treatment with amlodipine is effective in reducing tissue damage induced by ischemia, I/R, or both in ovaries.

• Authors: Zekai Halici, Mehmet Karaca, Osman Nuri Keles, Bunyamin Borekci, Fehmi Odabasoglu, Halis Suleyman, Elif Cadirci, Yasin Bayir, Bunyami Unal
• Journal: Fertility and sterility (Fertil Steril) Vol. 90 Issue 6 Pg. 2408-15 (Dec 2008) ISSN: 1556-5653 [Electronic] United States
• PMID: 18178199 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Amlodipine
• Nitric Oxide
• Nitric Oxide Synthase
• Superoxide Dismutase

Topics

• Amlodipine (pharmacology)
• Animals
• Antioxidants (pharmacology)
• Disease Models, Animal
• Female
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (metabolism)
• Ovarian Diseases (complications, drug therapy, metabolism, pathology)
• Ovary (drug effects, enzymology, pathology)
• Rats
• Rats, Wistar
• Reperfusion Injury (etiology, metabolism, pathology, prevention & control)
• Superoxide Dismutase (metabolism)
• Torsion Abnormality (complications, drug therapy, metabolism, pathology)