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Chaperone mediated solubilization of 69-kDa recombinant maltodextrin glucosidase in Escherichia coli.

AbstractAIMS:
To investigate the factors affecting expression and solubilization of Escherichia coli maltodextrin glucosidase in E. coli.
METHODS AND RESULTS:
Expression level and solubilization of the recombinant E. coli maltodextrin glucosidase was studied in E. coli at different temperatures, in presence of overexpressed GroEL, GroES and externally supplemented glycerol. Aggregation of maltodextrin glucosidase in the cytoplasm was partially prevented by the co-expression of GroEL and GroES, and using externally supplemented glycerol or lowering the culture temperature. Co-expression of GroEL and GroES or simultaneous presence of overexpressed GroEL, GroES and externally supplemented glycerol together resulted significant increase of the activity of maltodextrin glucosidase. The growth rate of E. coli was inhibited by the formation of inclusion bodies whereas the presence of overexpressed GroEL, GroES alone or together with glycerol enhanced the growth rate of E. coli substantially.
CONCLUSIONS:
The results indicated that lowering the temperature, use of GroEL, GroES and glycerol could be few controlling factors for the solubilization of recombinant aggregation-prone maltodextrin glucosidase in E. coli.
SIGNIFICANCE AND IMPACT OF THE STUDY:
Our study could help in developing the strategy for enhancing the production of soluble industrial enzymes and finding the therapeutic agents against protein misfolding diseases.
AuthorsS Paul, T K Chaudhuri
JournalJournal of applied microbiology (J Appl Microbiol) Vol. 104 Issue 1 Pg. 35-41 (Jan 2008) ISSN: 1365-2672 [Electronic] England
PMID18171380 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chaperonin 10
  • Chaperonin 60
  • Escherichia coli Proteins
  • Molecular Chaperones
  • Recombinant Proteins
  • Glycoside Hydrolases
  • malZ protein, E coli
  • Glycerol
Topics
  • Bioreactors (microbiology)
  • Chaperonin 10
  • Chaperonin 60
  • Escherichia coli (growth & development, metabolism)
  • Escherichia coli Proteins (chemistry)
  • Glycerol (pharmacology)
  • Glycoside Hydrolases (chemistry)
  • Industrial Microbiology
  • Molecular Chaperones
  • Protein Folding
  • Recombinant Proteins (metabolism)
  • Temperature

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